LOCUS       sequence-125186-        7715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q 
            intersectional method .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW54
FEATURES             Location/Qualifiers
     source          1..7715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1141..1161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3208..3219
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3933..3966)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(5234..5256)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5356..5375
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5457..5475)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5543..5647
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5648..5716,5717..6508)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5866..5885)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6679..7267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7168..7187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7421..7438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7555..7576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7591..7596,7597..7614,7615..7621)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7629..7645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7634..7656
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccactgact
       61 ggtgatccat ctcgaatttc tcgtttcaaa ttagtcgttg ctatatatat tgtcttatgt
      121 gtggaaaacg aataagaaaa attagagaaa gatgagagag caggggcaga atgaaaattt
      181 gcatggaaat tgaagaagat cactgctttt tccgatgaat gatgactaaa tagaatgagt
      241 tgagaggaaa ataatcataa ttctaatttt aattagaaat atgatatgga aagtaagcaa
      301 aaccacaagg cttttgttct gaacttattt ccgaaaaaaa aagtgaaact gaaagttaga
      361 attttcaaaa taaatattgc gagagtcaac cttcagaaaa attggcgaga aatcaaattt
      421 gaattctctg aaaatttagt taaaatacat atgttatcct tctttgtttt ttggccttag
      481 cctgccttca aggcgacctg agcctatgcg ggtgcctaat accgaaacac acataatttt
      541 ttttttttaa tctttaaata ctgtaacgag tcgcgagatc cagcgagacc catagaatat
      601 gttgagcgct gcaaacaatc gtataactct tgtccaaatt tttccactct gtatttttgc
      661 aagcagttca acaaatggtc atcattttta taacccatat ctatattcga atctgacata
      721 acaaataata ctaagcacat cctcccacat ctcttttcat tcaagttgcg caattcccct
      781 aataactatg gatggagggg ggaccgagcc caaagattat tcaaaacgat aacgactttt
      841 tcatgcggcc aacgaaaaat aaaacttttc tcatattctc ctatttccca tttcaagaat
      901 tacatcccca tgggacgttg aaatcacatc atattagtat atgttaattg ttcttttcac
      961 tggaatatcg ccacgccccc cttccggtgg ctcattggtt tcgagaagaa ggcggagccg
     1021 aagtgggttc ttctttcttt tttttgtggt agaagtctag caggattaag ctactattcc
     1081 attagaggat cttcttcttc caaaaagtga aaggcgcgcc tctagaggat ccaaaaaatg
     1141 ccaaagaaga agcgtaaggt cgcttctatg ccaccaaaac gcaagacact caatgccgct
     1201 gccgaagcca atgcccacgc tgatggccat gctgatggca atgctgatgg tcatgtcgct
     1261 aacactgcag caagcagcaa caacgcccgt tttgcggact tgaccaacat tgacacaccc
     1321 ggcctcggcc ctaccacgac gacgttactt gtcgagcccg ctcgttcgaa acgccagaga
     1381 gtctcgaggg cctgtgatca gtgtcgagct gcacgtgaaa agtgtgatgg aatccagccg
     1441 gcttgcttcc cctgtgtgtc gcagggccgg tcgtgtacct accaggccag tcccaagaag
     1501 cgaggagtcc agacgggcta catccgcact ctcgaactgg ctctggcttg gatgttcgag
     1561 aacgttgccc gcagcgagga cgccctccac aatcttttgg tccgtgatgc tggccagggc
     1621 agcgctctcc tggtcggcaa agactcgcct gctgcagaac gcctccatgc aagatgggcg
     1681 acgagtcgag tcaacaaaag catcacccgt cttctctcag gtcaggccgc acaagatcca
     1741 tctgaagacg gccaatcccc gtccgaagac ataaatgtcc aagatgccgg ggcaaagaca
     1801 tccgacttcc ctcatgcgcc tcacttgact ttctcggcgc ctaagtctag tacagctgag
     1861 acacgcactc taccaggccc ggtccgacca cctatttcgg caaacaccct ggaaaacaac
     1921 cttcagccag atggaaccgg gatagggaag ctaccaccca atcattggcg cctgctggat
     1981 atctactttt cctacacgca ttcttggctc cctatcctcg agaagaaaga catgtaccaa
     2041 gcattgtacc agtactctga acaaggctcg ttgcttccct ctgcgaatgt cgagtctggc
     2101 gttcatgccg agctttggag cgcgctcgcc ctggcgtcct tccaggctgc tgctactgct
     2161 gcatcgagtg ctacgggtcc agcttcagct gctcatggcc atgacaatgc catcaatcct
     2221 tcacctgcag acatatctga cacagcccga aagctcatac ctttggaaag cgggccgttc
     2281 caggttcagc actgcagagc gttgctgctt ctttgtctcg taagccttgg gcgggatgat
     2341 tgggagtctg cttggttgct ggttggcttt gcggtccgcg tcctacttgt tgttcgcacc
     2401 cagttgcctc ctgatgatga ccggccacga ccaagaatgc gtgcgctgct cgtcgcgtgc
     2461 ttcatcgtgg ataccattgt gtctatgaga cacaacgtgc cggcccatct caagccagac
     2521 gacattgcgg atctgccgtt acctgaagac ggtcaagatc aatgggagcc gtggacacca
     2581 tgtgagggct taggcggtga acacaccatg ctgcaaatgt tgaggaaccc ggcataccct
     2641 ttaagcacat tcaaccacct atatggcgtg accaagctgg ttgctttgga gcttctgcca
     2701 agaatacgaa catcttcaca gaacgctccc ttggagttca ggtcgcggtt gcagcaggta
     2761 atcggccaca attctccctt cagcgtcttt gtcctttccc aggatacagc atcggctttt
     2821 gtgcctactg cataccttac ccgtaccgtt tatttatggg cagctgcctt ttctgagcct
     2881 ctcaacgaac actactcgca tcttctgatc gaaactcttg atcagtatca gaagcggttt
     2941 ggtacatatg caatcccacc tctgatccct tctcttctag actcccttct tgctttaaag
     3001 aaacaatcac attcttcaga gcggcatcga aggcacttgg aagagctttt ccccgcctac
     3061 tcctccattt ggcctcgggg aggccgacac agcaatactg gcctccaacc catacgacaa
     3121 ggatccggat ctggatctgg atctggatct ggttccgctc ttaagaaaga gctgcaggca
     3181 aacaagaaag agctggctca gctgaagtgg gaactgcagg cactgaagaa agaactggct
     3241 cagtaagcta gcgtcgacgg taccgctgtc tcatcctact ttcacctagt taactgcttg
     3301 tcttaaaatc tatgcttctc tttagtatct aaaattttcc tagaagctta caagtatata
     3361 aatggtctct tctcaataaa ggttgtatat ttattcatct tattgaatct gccatttcct
     3421 cgtttttgcg agtttatata ccttccaatt ttctttctat tgtattttca acttctaatt
     3481 ttaattcagg gaaactgctt caacgcatca tggtctcaaa gggtgaagaa gataacatgg
     3541 caattattaa agagtttatg cgtttcaagg tgcatatgga gggatctgtc aatgggcatg
     3601 agtttgaaat tgaaggtgaa ggagaaggcc gaccatatga gggaacacaa accgcaaaac
     3661 taaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtaa
     3721 ctaaaggcgg accattacca ttcgcctggg acatcctctc tccacagttc atgtatggaa
     3781 gtaaagctta tgttaaacat ccggcagata taccagatta tttgaaactt tcattcccgg
     3841 agggttttaa gtgggaacgc gtaatgaatt ttgaagacgg aggagttgtt acagtgacgc
     3901 aagactcaag gtaagtttaa acagttcggt acgaagttcc tatactttct agagaatagg
     3961 aacttctaac taaccataca tatttaaatt ttcagcctcc aagatggaga atttatttat
     4021 aaagtcaaac ttcgaggaac gaatttcccc tcggatggac ctgttatgca gaagaagact
     4081 atgggatggg aagcttcaag tgaaagaatg taccctgaag acggtgctct taagggagag
     4141 attaaacaac gtcttaaatt gaaagatgga ggacattacg atgctgaggt aagtttaaac
     4201 atgattttac taactaacta atctgattta aattttcagg tgaagacaac ttacaaagcc
     4261 aaaaaaccag ttcagctgcc aggagcgtac aatgttaata ttaaactgga tatcacctcc
     4321 cacaacgagg attacactat cgttgagcaa tatgaaagag ctgaagggcg gcactcgaca
     4381 ggtggcatgg atgaattgta taagtaagag ctccgcatcg gccgctgtca tcagatcgcc
     4441 atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc
     4501 tttctccctg tgctcccacc ccctattttt gttattatca aaaaaacttc ttcttaattt
     4561 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     4621 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     4681 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt ttacttctga
     4741 taaatttttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     4801 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
     4861 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
     4921 ttatgaaatt aattttcctg cttttgcttt ttgggggttt cccctattgt ttgtcaagag
     4981 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     5041 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     5101 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     5161 aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     5221 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5281 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5341 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     5401 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa ttaagggcct
     5461 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5521 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5581 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5641 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5701 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5761 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5821 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5881 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5941 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6001 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6061 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6121 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6181 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6241 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6301 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6361 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6421 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6481 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6541 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6601 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6661 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6721 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6781 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6841 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6901 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6961 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7021 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     7081 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7141 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7201 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7261 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7321 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7381 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7441 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     7501 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7561 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7621 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctgggggt gggggggggg
     7681 ttgggggtgg ggtgttgttg gggtgtgttt tgtgt
//
