LOCUS       sequence-125192-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry (codon adapted for Bacillus subtilis); under 
            control of Zinc inducible promoter PZn. Plasmid integrates in S. 
            pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01_mCherry
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgaaacgcg cgaggcaggc taaacctgct gctactgctg cttggcttgc cgcactacct
       61 gtatttggta actgggcttt attagtttga tcagcttcac ttgaatcaat tggttttgta
      121 tctgcttttt ctgacacttg tggtttttta gcttcttgag ctactggttt ggttccaacc
      181 aagacgatgc ggtctgtcgg aacttctacc acttcacgga gtttttcttc cttacttcca
      241 tcaggattaa tcactgtaaa gatacgttct tttccaactt ttccttcttg ttctacacga
      301 gtttcaccta gatacagtgt tgaatctttt ttctcaactg tcttgtaggc caaatctttt
      361 tcaacaaatt cgagttttgg aagatcttct tgtacagcag aaactggttt ttccttagtc
      421 aactggatac ggtattcctt gacttgtttt ccactttctg aaacgaggcg aacaagtact
      481 ggaaggctat cttctccact atctaccaca gttgaagcta cttgattgtt ttcttcaact
      541 gagacttttg gccgttgacc tttataggta atttgatagt cttgacgatt ttcagcgaaa
      601 tcagcaagtt cttttccatc tacaagaatc tttgattgag tgctttcttg aggcaattca
      661 cttggtgcaa ggaaggtcat ctcaatcatc gcaacaccgc tcttgtcagc tttacgctcc
      721 atacgccatc tcatgacttt ggctttgaca gctttaaatg ttacgttgat ttcatcacca
      781 gaaatagtac ataatggatt tccttacgcg aaatacgggc agacatggcc tgcccggtta
      841 ttattatttt tgacaccaga ccaactggta atggtagcga ccggcgctca gcgatagcgc
      901 tatcaaaaga atcttgcttg gcaaggttct tttttttgtc ttttcaaaaa agcacgaaaa
      961 aagcacccat aaggtaaact tttgagtgct ttgctataaa tacgcttcac agtttcttct
     1021 tcatttttaa caaacaaaaa acccagctca ttgagctggg tttaagctgg cttggcggcg
     1081 tcctagtact gcagaattgc cgaccttgac tagtgctcat tattatttgt aaagctcatc
     1141 cattccgcc
//
