LOCUS       sequence-125194-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  It encodes a fluorescent protein that can be fused to a gene of 
            interest using SEC CRISPR strategy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  TITLE     Hobert plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUA97
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             287..307
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(325..358)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             368..397
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aggtcgaaac gacatggccc tgaagctcgt cggcggtatc catctcatcg caaacgctaa
       61 gaccacatat agatccaaga aaccggtaag tttaaacatg attttactaa ctaactaatc
      121 tgatttaaat tttcaggcta agaaccttaa aatgccggga gtctactatg ttgactacag
      181 actggaacgg atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggttgcagt
      241 tgccagatac tgcgacctcc cgagcaaact gggtcacaag cttaatgaga atctgtactt
      301 tcaatccgga aaggtaagtt taaaataact tcgtatagca tacattatac aaagttattt
      361 tcagggagag caaaagctta tctccgagga ggacctctaa ggatgatcga cgccaacgtc
      421 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
      481 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
      541 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
      601 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
      661 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
      721 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaacta
      781 gctgccctcg attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt
      841 cttctctggt tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca
      901 cattcctcac ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc
      961 tgcgtctcgc acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact
     1021 tgcggaaaga tttcctaata aaaggaga
//
