LOCUS       sequence-125196-       11723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteroides coprophilus Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11723)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX388 Bacteroides coprophilus Cas9
FEATURES             Location/Qualifiers
     source          1..11723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1086..1389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1544..1564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1615..1634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1634..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1661..1770
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1772..1796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1825..1851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1855..1875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6447..6464)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(6469..6493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6495..6971
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6694..6713)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     terminator      6972..7019
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7112..7540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7199..7218)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7409..7430
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7549..7569)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7554..7883
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7734..7869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7796..7815
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7950..8744
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8004..8023)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8614..8633
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8918..9039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8955..8974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9009..9028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    9083..9138
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9137..9156)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      9226..9273
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     9286..9991
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           9374..9746
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(9403..9991)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(10000..10033)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(10201..10788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10280..10299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10801..11592,11593..11661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11424..11443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgaag aaaacactgg gactggacct ggggactaac
     1921 agcattgggt gggccgtcat caactcaaac atcgactcag aaggaaaaga aaagctggtg
     1981 gggatcagct cctgcggaag ccggatcatt cctatggacg ctaccacact gggagatttc
     2041 ggaaagggca acactaaaag tccagtggca gagcgaaccc gactgcgggg cattcggaga
     2101 ctgctggaaa ggtcactgct gaggcgcgag cgactgcacc gagtgctgtc agtcatgggg
     2161 ttcctgccag agcattacgc tagccagctg gaccgctatg gaaagtttct gccagaaacc
     2221 gagcccaagc tggcatggta caaagacgat agcggcaggt atcagttcct gtttcagaag
     2281 tccttccacg agatgctgga ggactttcga cagcatcagc cagaactggt ggcaggagag
     2341 aagaaaatcc cttacgattg gacaatctac tatctgcgga agaaagcact gtctcaggag
     2401 atcactaagg aggaactggc ctggattctg ctgaatttca accagaaacg cggctactat
     2461 cagctgcgag gggaggaaga gcaggaagag aacaataaga gcgtggagta tcacgccctg
     2521 aaagtggtga gcgtcgagga ctctggagaa agaaagggca aagatatctg gtacaatgtg
     2581 actctggaga acggatgggt ctatcgacgg gctagcaaca ttcccctgga ctggaccggc
     2641 aaggtgaaag agttcgtggt cactaccgag ctggacgatg ccgggaaccc aaagaaagat
     2701 aaggaaggaa atgtgaaaag atcctttagg gcaccaaagg aggacgattg ggggctgctg
     2761 aaaactagga cccaggctca gatcgacgaa tccggcaaga ccgtgggaac ttacatctac
     2821 gagtctctgc tgtgtatgcc caaccagaag atccggggaa aactggtgag gaccattgaa
     2881 cgcaagtact ataaagacga gctgagacag atcctggtga agcagagcga gttccacgcc
     2941 gctctgcagg atcataatct gctgctgtcc tgtatcgaag agctgtaccc taacaatgag
     3001 gcccacagaa ggctgctgag cgccagcagc ttcatctact tcctgatcga ggacattctg
     3061 ttttatcagc gcccactgaa gtcccagaaa gggctgatcg ataactgccc ctacgagtct
     3121 cacatctaca aggataagaa agatggaagt ctgcaccatg tgcctctgaa gtgtgtcagc
     3181 aaatcccatc cactgttcca ggaatttcgc ctgtggcagt tcctgtctaa cctgcgaatc
     3241 taccagagag agaggatgat ggacggcagt ctgaaactgg acgtggatgt cacccgggag
     3301 ttcctgccct cagaagagga ctacgtgaag ctgtttgatt ggctgaatga gagaaaggaa
     3361 atctctcaga aattcctgct ggcttataaa ccttttgggc tgaagaaaaa cgaacaggca
     3421 aattacagat ggaactatgt ggaggacaag agctacccct gcaacgagac acgggcagaa
     3481 atcaagagca gactgtccaa agccggagtg cctgaagagt ttctgactga agagaaggaa
     3541 gaggccctgt ggcacatcct gtattctatt agtgataaga aagagctgac taaggctctg
     3601 ggcaccttcg cagccaaaaa ctgtctgaat gagtctttcg tggaagtctt tgccaagatc
     3661 cccccttttg agtcaaacta cgctgcatat agcctgaagg ctattaggaa actgctggca
     3721 ctgatgcgca tggggaagta ctggaatgaa caggccatcg acaggcagac tcgcgatcga
     3781 atcgagaaaa ttctgaccgg agagtatgac gaaacaatcc ggagcagagt gagggagaag
     3841 gcaatgctgc tgaccgatat tagcagcttc cggggcctgc ctctgtggct ggcctgttac
     3901 atcgtgtatg accgccactc agagagcaag gaactggtca aatgggagac accagccgac
     3961 atcgatcatt tcctgtccaa gtttaaacag aacagcctgc ggaaccccat cgtggaacag
     4021 gtcattacag agtccctgcg cactgtgcga gacatttgga agcaggaggg aaaaatcgat
     4081 gagattcacg tggaactggg ccgggagatg aagaaccctg caaaagagcg cgcccgaatt
     4141 acagctcagg tgcaggaaaa tgagaacact aatctgagaa tcaaggctct gctggcagag
     4201 ttcatgaacc ccgaatttga gattgaaaat gtgcatccat actcacccgg ccagcaggaa
     4261 atcctgcgga tctacgagga cggcgtgctg agcgggatcg ctgagaagga tctgcctgac
     4321 gatatcacag caattctgaa gaaattccga gaaaacgacg tgaagaaacg gccaacaact
     4381 agcgaagtcc tgcggtacaa actgtggctg gagcagcggt acagatcccc atataccgga
     4441 agagtgatcc ccctgggcaa gctgttcaca cctgcttacg agatcgaaca cgtgattccc
     4501 cagagccggt attttgacga ttccatctct aacaaagtga tctgcgaaag tgccgtcaat
     4561 aagctgaaag ataactgtct gggctatgag ttcatcaaga aacattccgg ggagatggtg
     4621 gaactgggga atggagagac agtgcccgtg ttcagcgtgg aagagtacga acggttcgtc
     4681 aaggagtctt acttcggcaa cagtaagaaa atgaagaaac tgctgctgga ggacatccca
     4741 gatagcttca ttgagagaca gctgaatgac agtcgataca tctcacgggt ggtcacatct
     4801 ctgctgagta acctggtgtg cgaagaggga gagcaggatg gcctgtccaa gaatgtgatc
     4861 gtctgtaccg gcgggattac agacaggctg aagaaagatt ggggagtgca ggaagtctgg
     4921 aaccgcatca ttctgcctcg gttcctgaga ctgaatgaga tcaccggacg gacagacttt
     4981 acaagtactt cagtgaacgg ccacctgctg cctgccctgc cactgtacct gcagaagggc
     5041 tttaataaga aaagaattga ccataggcac catgccatgg atgctatcgt gattgcctgc
     5101 gctaaccgga atatcgtcaa ctacctgaac aattcctctg ctagaaagaa cagcgaaatt
     5161 agccgatatg acctgcagcg gctgctgtgt gagaaggtga aaaccgatgc caacggcaat
     5221 tacaagtgga tcctgaggaa accatgggag acattcaccc aggatgtgta tgccgctctg
     5281 acaaacatcg tggtcagctt taagcagaat ctgcgcgtga ttaatcgaac cacaaactac
     5341 tatcagcact acaacgaggc aggggaaaag cgcatgatcc ctcagaccaa aggcgacaga
     5401 tgggccatta gaaagccaat gcataaagat actgtgtatg gcgaagtcaa tctgagaaag
     5461 gagaaaaccc tgccactgag ggacgtggtc aagaacccca gtatcgtggt cgataaatca
     5521 ctgaagaaca agctgtacga gctgctgaag agccagtatg acctgaaggc aatcgccaaa
     5581 tacttcgaga cacaccagga cgtgtgggca gatgtcaacc tgaagaaaat taaggtgtac
     5641 tacttcacta aggagacaaa cgaaagattc tttgccacta ggaagagcct ggacccatcc
     5701 tttgatcaga agaaaatcga agaggaagtg actgataccg gcgtccagaa gattctgctg
     5761 caccatctgc agcagaacaa taacgaccct gatatggcct tttccccaga cggcatcgat
     5821 aggatgaacc agaatatgac cattctgaat gacgggaagt ggcaccagcc catctacaaa
     5881 gtgcgcacat atgaaaaggc agataaattc gccgtcggcg agtctgggaa caaggccaag
     5941 aaatttgtgg aagcagccaa aggcaccaat ctgttctttg ccgtgtacga gagtgtccag
     6001 gaggacgaag ctatcgggaa gcaggtctgc aaacggacat tcgccactat ccccctgaac
     6061 gaagtgatca agagaaagaa acagggcctg cccgctgcac ctgaggacct gaacgggaat
     6121 ctgcccaagt ttgtgctgag ccctaacgat ctggtctacc tgcccaccga ggaagagagg
     6181 aatagttcac gcatcattca gcctctggac agggagcgca tctataagat ggtgagctcc
     6241 tctgggagtc agtgcttctt tatcaaagtg ttcgtcgcca attcaatttg ggataagaac
     6301 gaatacagca gcctgaacaa gatggagagg gctattacaa acgaaatgat caaggagatt
     6361 tgtgtgccta tcaaaattga ccgcctgggc aatgtcagcc tgatccagat ttaagaattc
     6421 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
     6481 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     6541 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     6601 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     6661 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     6721 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     6781 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     6841 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     6901 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     6961 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
     7021 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7081 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     7141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7561 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7621 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7681 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7801 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7861 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7921 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7981 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     8041 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8101 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     8161 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8221 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8281 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8341 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8401 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     8461 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     8521 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8581 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8641 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8701 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8761 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8821 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8881 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
     9061 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     9121 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     9181 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
     9241 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
     9301 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
     9361 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
     9421 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
     9481 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
     9541 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
     9601 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
     9661 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
     9721 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
     9781 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
     9841 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
     9901 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
     9961 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
    10021 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10201 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10381 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    11101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    11161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    11221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11701 ttattgtctc atgtccgcgc gtt
//
