LOCUS       sequence-125197-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ142
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     79..101
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     93..110
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     94..110
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        120..138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(186..206)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(186..204)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(225..241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(238..260)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    249..265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(273..279,280..297,298..303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    318..339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(404..503)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(521..539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(556..574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(579..595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
       61 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
      121 aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc ctccagcttt
      181 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
      241 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      301 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      361 gctttccacc ggtggtctct ggacactagt aagggcgaat tcgacccagc tttcttgtac
      421 aaagttggca ttataaaaaa taattgctca tcaatttgtt gcaacgaaca ggtcactatc
      481 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
      541 atggtcatag catcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
      601 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      661 gcctcctctg gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
      721 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
      781 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga a
//
