LOCUS       sequence-125201-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry (codon adapted for Bacillus subtilis); under 
            control of Zinc inducible promoter PZn. Plasmid integrates in S. 
            pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01_mCherry
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgtcctgtg gatctatcga tgcatgcagc cggtaccatc acaagcactt tgggacgttc
       61 tcccttagtg cttttttgat ttctcatagg ccggcctgtt agtcatatgg acacttaagg
      121 caaattgttc agaactgaat aaagctgacg ttttgcttct atcctttctt tgagttttag
      181 tggataatga taatgaacaa ggtgttcata aatctattat aacaagaatt ccaatcccgg
      241 gtaaagcttc cagcggccgc ataggatcca ttgatatcat attctagata ctgattaact
      301 aataaggagg acaaacatga gcaaaggaga agaagataac atggcaatca tcaaagaatt
      361 tatgcgtttc aaagttcaca tggaaggttc tgtaaacgga cacgaatttg aaattgaagg
      421 tgaaggtgaa ggccgtcctt atgaaggaac acaaacggca aagctgaaag taacaaaagg
      481 cggaccgctt ccgtttgcat gggatatcct ttctccgcaa ttcatgtacg gttcaaaagc
      541 atacgtgaag catccggctg atattcctga ttatttgaag ctgtcattcc ctgaaggctt
      601 caaatgggag cgtgtgatga actttgaaga tggcggtgtt gttactgtta ctcaagattc
      661 aagccttcaa gacggtgaat ttatttacaa agtgaagctg cgcggaacaa acttcccatc
      721 tgacggacct gtcatgcaaa agaaaacaat gggctgggaa gcaagctctg aacgcatgta
      781 tccagaggac ggtgctttaa aaggagaaat caaacagcgt ttgaagctga aagacggcgg
      841 acactatgac gctgaagtga aaacaactta caaagcgaaa aagccggttc agcttccagg
      901 tgcttacaac gtaaacatca aacttgatat tacaagccac aatgaagatt atacgattgt
      961 tgaacaatat gaacgcgctg aaggccgtca ttcaactggc ggaatggatg agctttacaa
     1021 ataataatga gcactagtca aggtcggcaa ttctgcagta ctaggacgcc gccaagccag
     1081 cttaaaccca gctcaatgag ctggg
//
