LOCUS       sequence-125207-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 3rd gRNA under AtU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ133B
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(292..367)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgt
       61 aactcacgtt aagggatttt ggtcatgggt ggctcgaggg tctccctggc acaatgtttt
      121 aatttaggct cattatagat tctagttcca aaattcaaga attatctcta aatcagtcat
      181 gaaagacatt cacatggatg ttggtttgtt ctaggttttc aatatctaca atgatcgata
      241 tacaaattta agtttatggc aaaaaaaaga aaaaaggaaa gggaaaaaaa agcaccgact
      301 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
      361 ctaaaacaga gacggtcgac tcagactaga tctcgtctct gaccaatggt gctccctcag
      421 tgttatatat acaacaagat ttgtttagat ttgagcgatg tgggagaaat taggagctta
      481 tatgaaacgc atacatgggt actgggcatg ggtattagtg aatatatgga cgatagccca
      541 ttaaattaca taaaaagccc acttatctta ttgctcgata taagctccgt atataaacgg
      601 aagttgatcc taaaagttca cttgtttcgt ccaacatagc caaaacagat gttcttaaac
      661 ccagacgcca tttgtttgat tcagttatcc atcacaggct ga
//
