LOCUS       sequence-125208-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector; 3rd gRNA under AtU3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, 
            Zheng X, Hsieh TF, Qi Y
  TITLE     A CRISPR/Cas9 toolbox for multiplexed plant genome editing and 
            transcriptional regulation.
  JOURNAL   Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
  PUBMED    26297141
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Aug 21. pii: pp.00636.2015."
COMMENT     Sequence Label: pYPQ133B
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(354..373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(355..373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(378..394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(378..394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        842..917
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
       61 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
      121 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
      181 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      241 tcttgcggag aactgtgaat gcgcaaacca acccttgatc ggggaagaac agtatgtcga
      301 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat atcccctata
      361 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      421 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag aggtctcagg
      481 actttacttt aaatttttct tatggctcag cctgtgatgg ataactgaat caaacaaatg
      541 gcgtctgggt ttaagaacat ctgttttggc tatgttggac gaaacaagtg aacttttagg
      601 atcaacttcc gtttatatac ggagcttata tcgagcaata agataagtgg gctttttatg
      661 taatttaatg ggctatcgtc catatattca ctaataccca tgcccagtac ccatgtatgc
      721 gtttcatata agctcctaat ttctcccaca tcgctcaaat ctaaacaaat cttgttgtat
      781 atataacact gagggagcac cattggtcag agacgagatc tagtctgagt cgaccgtctc
      841 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      901 tggcaccgag tcggtgcttt tttttccctt tccttttttc ttttttttgc ca
//
