LOCUS       sequence-125210-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under
            control of Zinc inducible promoter PZn. Plasmid integrates in S. 
            pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01_tagRFP
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgtcctgtg gatctatcga tgcatgcagc cggtaccatc acaagcactt tgggacgttc
       61 tcccttagtg cttttttgat ttctcatagg ccggcctgtt agtcatatgg acacttaagg
      121 caaattgttc agaactgaat aaagctgacg ttttgcttct atcctttctt tgagttttag
      181 tggataatga taatgaacaa ggtgttcata aatctattat aacaagaatt ccaatcccgg
      241 gtaaagcttc cagcggccgc ataggatcca ttgatatcat attctagagg aggaaaatta
      301 atgtcagaac ttatcaagga aaatatgcac atgaaattgt acatggaagg aactgtcaat
      361 aatcaccact ttaaatgtac ctcagaagga gaaggaaaac catatgaagg tactcaaacc
      421 atgcgtatta aggttgtcga aggtggacca cttccttttg cttttgatat tcttgcaaca
      481 tcttttatgt acggatcacg tacctttatc aatcatacac aaggtatccc agatttcttt
      541 aaacagtctt ttcctga
//
