LOCUS       sequence-125212-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBS/U6 containing shRNA to mouse SADBs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pBS/U6/RNAi SadBs shRNA mouse
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..25)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9..25)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(62..82)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(62..80)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(101..117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(101..117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(114..136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    125..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(149..155,156..173,174..179))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(332..349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(583..602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
       61 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
      121 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      181 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      241 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      901 cagagttctt gaagtg
//
