LOCUS       sequence-125213-         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBS/U6 containing shRNA to mouse SADBs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pBS/U6/RNAi SadBs shRNA mouse
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(11..27)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11..27)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(44..63)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(45..63)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(73..90)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(73..89)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..104)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      231..611
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(318..337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     528..549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcggcgtcg gatccactag ttctagagcg ggtacccaat tcgccctata gtgagtcgta
       61 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      121 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      181 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      241 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      301 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      361 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      421 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      481 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      541 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      601 gccgatttcg gcctattggt taaaaaatga gctgg
//
