LOCUS       sequence-125214-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Ward J
  TITLE     Ward lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJW1328
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(401..750)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     756..778
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     770..787
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     771..787
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    795..815
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gacacatgca gctcccggag acggtcacag ctttgtctgt aagcggatgc cgggagcaga
       61 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      121 gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      181 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
      241 gggcgatcgg tgcgggcctc ttcgctatta cgccagaaat ttcacaaaaa aaagcaccga
      301 ctcggtgcca ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgtt
      361 tccagcatag ctcttaaaca tgttcatcaa gttattcatc aagacatctc gcaataggag
      421 gtgtttatat aggggcaaca caagaggcgg cagacatctc gtgagtttga cagagtagta
      481 taaagagtgt gtgtgagagg gtgtaggcat ttcataacat attttttgtg gaataatttt
      541 ggaaaaaagg tgttttttta ataatagctt ttataagagt ttctcaaaaa ttgcatgctt
      601 tttttgaaaa ggttgacaaa ttatagtagt actaaagttg ctattttcag gtctcagcag
      661 aaaattcaca actaaaaata aaatttgcaa aacagagtgt gcgcctttaa ggtgtacagt
      721 tttttattcc tttattgcta gttttttttg gttttcccag tcacgacgtt gtaaaacgac
      781 ggccagtgaa ttatcaacta tgtataataa agttgtgaga ct
//
