LOCUS       sequence-125216-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Ward J
  TITLE     Ward lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJW1328
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    337..358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(373..378,379..396,397..403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    411..427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     416..438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     435..451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     435..451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        469..487
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     469..486
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(633..653)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(661..678)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(661..677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..692)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      301 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      361 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      421 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca
      481 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
      541 ccgccagtgt gctggaattc gcccttattg tgttcgttga gtgacccaac aaaaagagga
      601 aactttattg tgccgccaag aaaaaagtct cacaacttta ttatacatag ttgataattc
      661 actggccgtc gttttacaac gtcgtgactg ggaaaacca
//
