LOCUS       sequence-125219-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Zhang L, Ward JD, Cheng Z, Dernburg AF
  TITLE     The auxin-inducible degradation (AID) system enables versatile 
            conditional protein depletion in C. elegans.
  JOURNAL   Development. 2015 Nov 9. pii: dev.129635.
  PUBMED    26552885
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Nov 9. pii: dev.129635."
COMMENT     Sequence Label: pJW1354
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..268
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             269..289
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             296..361
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(476..494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(477..494)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(512..528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(525..547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    536..552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(560..566,567..584,585..590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    605..626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(743..760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattcgcc ctttcccagc
       61 aatccaactg ccaaccccac aagccacata taccgcagta ccttatatgg catcaggatc
      121 cggaggtggc gggatgccta aagatccagc caaacctccg gccaaggcac aagttgtggg
      181 atggccaccg gtgagatcat accggaagaa cgtgatggtt tcctgccaaa aatcaagcgg
      241 tggcccggag gcggcggcgt tcgtgaagga gaatctgtac tttcaatccg gaaaggacta
      301 caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa
      361 gtaatgtctc tgtcatagga gctgaaaact tccaataagg gcgaattcca gcacactggc
      421 ggccgttact agtggatccg agctcggtac caagcttgat gcatagcttg agtattctat
      481 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcag
//
