LOCUS       sequence-125220-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Zhang L, Ward JD, Cheng Z, Dernburg AF
  TITLE     The auxin-inducible degradation (AID) system enables versatile 
            conditional protein depletion in C. elegans.
  JOURNAL   Development. 2015 Nov 9. pii: dev.129635.
  PUBMED    26552885
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Nov 9. pii: dev.129635."
COMMENT     Sequence Label: pJW1354
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..179)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(186..206)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(207..338)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     primer_bind     complement(491..510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(492..510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(517..534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(517..533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(526..548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tactcaagct atgcatcaag cttggtaccg agctcggatc cactagtaac ggccgccagt
       61 gtgctggaat tcgcccttat tggaagtttt cagctcctat gacagagaca ttacttgtca
      121 tcgtcatcct tgtaatcgat atcatgatct ttataatcac cgtcatggtc tttgtagtcc
      181 tttccggatt gaaagtacag attctccttc acgaacgccg ccgcctccgg gccaccgctt
      241 gatttttggc aggaaaccat cacgttcttc cggtatgatc tcaccggtgg ccatcccaca
      301 acttgtgcct tggccggagg tttggctgga tctttaggca tcccgccacc tccggatcct
      361 gatgccatat aaggtactgc ggtatatgtg gcttgtgggg ttggcagttg gattgctggg
      421 aaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
      481 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
      541 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      601 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      661 atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
      721 ggatgtacag agtgatatta ttgacac
//
