LOCUS       sequence-125227-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For protein expression of CYP2C19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Goldstein CYP2C9 and CYP2C9 in pOri+ for bacterial expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CYP2C19
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(146..151,152..169,170..176)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    184..200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(255..260,261..276,277..283)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    291..307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(350..355,356..371,372..378)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    386..402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg atggtgtcct
       61 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
      121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      181 gaattgtgag cggataacaa tttcacacag gaaacaggat cgatccatcg atgagcttac
      241 tccccatccc cctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg
      301 ataacaattt cacacaggaa acaggatcag cttactcccc atccccctgt tgacaattaa
      361 tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagg
      421 atccatcgat gcttaggagg tcatatggct ctgttattag cagtttttct ctgtctctca
      481 tgtttgcttc tcctttcaat ctggagacag agctctggga gaggaaaact ccctcctggc
      541 cccactcctc tcccagtgat tggaaatatc ctacagatag atattaagga tgtcagcaaa
      601 tccttaacca atctctcaaa aatctatggc cctgtgttca ctctgtattt tggcctggaa
      661 cgcatggtgg tgctgcatgg atatgaagtg gtgaaggaag ccctgattga tcttggagag
      721 gagttttctg gaagaggcca tttcccactg gctgaaagag ctaacagagg atttggaatc
      781 gttttcagca atggaaagag atggaaggag atccggcgtt tctccctcat gacgctgcgg
      841 aattttggga tggggaagag gagcattgag gaccgtg
//
