LOCUS       sequence-125229-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); 
            under control of Zinc inducible promoter PZn. Plasmid integrates in 
            S. pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01_mOrange2
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgtcctgtg gatctatcga tgcatgcagc cggtaccatc acaagcactt tgggacgttc
       61 tcccttagtg cttttttgat ttctcatagg ccggcctgtt agtcatatgg acacttaagg
      121 caaattgttc agaactgaat aaagctgacg ttttgcttct atcctttctt tgagttttag
      181 tggataatga taatgaacaa ggtgttcata aatctattat aacaagaatt ccaatcccgg
      241 gtaaagcttc cagcggccgc ggaggaaaat taatgtctaa gggagaagaa aataatatgg
      301 ctatcatcaa ggaatttatg cgttttaagg tccgtatgga aggttcagtc aatggtcacg
      361 aatttgaaat tgaaggtgaa ggagaaggtc gtccatacga aggttttcaa actgctaaat
      421 tgaaggtaac caaaggtgga ccattacctt ttgcatggga tattttgtca ccacagttta
      481 catacggaag taaagcatat gttaagcatc cagctgatat tcctgattac tttaaattga
      541 gttttcctga aggttttaaa tgggaacgtg tcatgaattt tgaagatggt ggagttgtca
      601 ccgtaacaca agattcttca ttgcaggatg gagaatttat ctacaaggtt aaattgcgtg
      661 gaaccaattt tccatctgat ggtcctgtca tgcaaaagaa aacaatgggt tgggaagcaa
      721 gttctgaacg tatgtaccct gaagatggag cccttaaagg tgaaatcaag atgcgtttga
      781 aacttaagga tggtggacat tacacttctg aagtcaagac aacttacaag gccaaaaagc
      841 cagtacagct tcctggagct tacatcgttg gtatcaaatt ggatattaca tcacacaatg
      901 aagattacac tatcgttgaa caatatgaac gtgcagaagg tcgtcactct actggtggta
      961 tggatgaact ttacaagtaa taataaggat cctaactcga gaaagaattc atgagactag
     1021 tcaaggtcgg caattctgca gtactaggac gccgccaagc cagcttaaac ccagctcaat
     1081 gagctgggtt tt
//
