LOCUS       sequence-125230-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); 
            under control of Zinc inducible promoter PZn. Plasmid integrates in 
            S. pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01_mOrange2
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaccgaaac gcgcgaggca ggctaaacct gctgctactg ctgcttggct tgccgcacta
       61 cctgtatttg gtaactgggc tttattagtt tgatcagctt cacttgaatc aattggtttt
      121 gtatctgctt tttctgacac ttgtggtttt ttagcttctt gagctactgg tttggttcca
      181 accaagacga tgcggtctgt cggaacttct accacttcac ggagtttttc ttccttactt
      241 ccatcaggat taatcactgt aaagatacgt tcttttccaa cttttccttc ttgttctaca
      301 cgagtttcac ctagatacag tgttgaatct tttttctcaa ctgtcttgta ggccaaatct
      361 ttttcaacaa attcgagttt tggaagatct tcttgtacag cagaaactgg tttttcctta
      421 gtcaactgga tacggtattc cttgacttgt tttccacttt ctgaaacgag gcgaacaagt
      481 actggaaggc tatcttctcc actatctacc acagttgaag ctacttgatt gttttcttca
      541 actgagactt ttggccgttg acctttatag gtaatttgat agtcttgacg attttcagcg
      601 aaatcagcaa gttcttttcc atctacaaga atctttgatt gagtgctttc ttgaggcaat
      661 tcacttggtg caaggaaggt catctcaatc atcgcaacac cgctcttgtc agctttacgc
      721 tccatacgcc atctcatgac tttggctttg acagctttaa atgttacgtt gatttcatca
      781 ccagaaatag tacataatgg atttccttac gcgaaatacg ggcagacatg gcctgcccgg
      841 ttattattat ttttgacacc agaccaactg gtaatggtag cgaccggcgc tcagcgatag
      901 cgctatcaaa agaatcttgc ttggcaaggt tctttttttt gtcttttcaa aaaagcacga
      961 aaaaagcacc cataaggtaa acttttgagt gctttgctat aaatacgctt cacagtttct
     1021 tcttcatttt taacaaacaa aaa
//
