LOCUS       sequence-125239-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CUC2 expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, 
            Baena-Gonzalez E, Wang JW, Weigel D
  TITLE     Temporal control of leaf complexity by miRNA-regulated licensing of 
            protein complexes.
  JOURNAL   Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 
            10.1016/j.cub.2014.09.058. Epub 2014 Nov 6.
  PUBMED    25448000
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.09.058"; journalName: "Curr Biol"; date: 
            "2014-11-17- 17"; volume: "24"; issue: "22"; pages: "2714-9"
COMMENT     Sequence Label: JW624
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             168..608
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     619..638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        619..637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             657..686
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc ttttgtttcc
       61 tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata tttcaagcta
      121 taccaagcat acaatcaact ccaagcttga agcaagcctc ctgaaagatg aagctactgt
      181 cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa
      241 aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca
      301 aaaggtctcc gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg
      361 aacagctatt tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt
      421 ctttacagga tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag
      481 atgccgtcac agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc
      541 atagaataag tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga
      601 ctgtatcgcc ggaatttgta atacgactca ctatagggcg agccgccatc atggaggagc
      661 agaagctgat ctcagaggag gacctgcata tggccatgga ggccgaattc atggacattc
      721 cgtattacca ctacgaccat ggcggagaca gccaatatct tccaccgggt ttcaggtttc
      781 atcccacgga cgaagagctc atcactcatt accttctccg caaagtcctc gacggttgct
      841 tctcaagccg tgccatcgca gaagttgatc tcaacaagtg tgagccttgg caacttcccg
      901 ggagagctaa gatgggagag aaagaatggt acttctttag cctccgtgac cggaagtatc
      961 cgacgggact gagaactaac agagcaactg aggctggtta ctggaaagct accggaaa
//
