LOCUS       sequence-125247-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under
            the inducible BAD promoter and a single copy of the endonuclease 
            cleavage site which is readily extendable to multiple copies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Chen Z, Zhao H
  TITLE     A highly sensitive selection method for directed evolution of homing
            endonucleases.
  JOURNAL   Nucleic Acids Res. 2005 Oct 6;33(18):e154.
  PUBMED    16214805
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-10-6"; volume: "33(18)"; pages: "e154"
COMMENT     Sequence Label: p11-LacY-wtx1
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    181..202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(217..222,223..240,241..247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     260..282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     279..295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(311..328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(311..327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
       61 gtgcggtatt tcacaccgca tacgtacgat ttaaataggc ctgactcact atagggagac
      121 cggaattccc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      181 taatgtgagt tagctcactc attagggacc ccgggcttta cactttatgc ttccggctcg
      241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      301 ttacggattc actggccgtc gttttacaac gtcgtgactg gtaaaacccg ggcgttaccc
      361 aacttaatcg ccttgcagca catccccctt tcgccagcag gcgtaataag gaaaggatcc
      421 atgtactatt tgaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt
      481 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgatat caaccatatc
      541 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa
      601 ccgctgtttg gtctgctttc tgacaaactc ggtctacgca aatacctgct gtggattatt
      661 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgctgcag
      721 tacaacattt tagtaggatc gattgttggt ggtatttatc taggctttag ttttaacgcc
      781 ggtgcgccag cagtagaggc atttattgag aaagtcagcc ggcgcagtaa tttcgaattt
      841 ggtcgcgcgc ggatgtttgg cagtgttggc tgggcgctgg ttgcctcgat tgtcgggatc
      901 atgttcacca ttaataatca gtttgttttc tggctgggct ctggcagttg tctcatcctc
      961 gccgttttac tctttttcgc caaaacggac gcgccctcga gtgccacggt tgccaatgcg
     1021 gtaggtgcca accattcggc attt
//
