LOCUS       sequence-125250-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under
            the inducible BAD promoter and a single copy of the endonuclease 
            cleavage site which is readily extendable to multiple copies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Chen Z, Zhao H
  TITLE     A highly sensitive selection method for directed evolution of homing
            endonucleases.
  JOURNAL   Nucleic Acids Res. 2005 Oct 6;33(18):e154.
  PUBMED    16214805
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-10-6"; volume: "33(18)"; pages: "e154"
COMMENT     Sequence Label: p11-LacY-wtx1
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggccgtcgt tttacaacgt cgtgactggt aaaacccggg cgttacccaa cttaatcgcc
       61 ttgcagcaca tccccctttc gccagcaggc gtaataagga aaggatccat gtactatttg
      121 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
      181 gcctacttcc cgtttttccc gatttggcta catgatatca accatatcag caaaagtgat
      241 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
      301 ctgctttctg acaaactcgg tctacgcaaa tacctgctgt ggattattac cggcatgtta
      361 gtgatgtttg cgccgttctt tatttttatc ttcgggccac tgctgcagta caacatttta
      421 gtaggatcga ttgttggtgg tatttatcta ggctttagtt ttaacgccgg tgcgccagca
      481 gtagaggcat ttattgagaa agtcagccgg cgcagtaatt tcgaatttgg tcgcgcgcgg
      541 atgtttggca gtgttggctg ggcgctggtt gcctcgattg tcgggatcat gttcaccatt
      601 aataatcagt ttgttttctg gctgggctct ggcagttgtc tcatcctcgc cgttttactc
      661 tttttcgcca aaacggacgc gccctcgagt gccacggttg ccaatgcggt aggtgccaac
      721 cattcggcat ttagccttaa gctggcactg gaactgttca gacagccaaa actgtggttt
      781 ttgtcactgt atgttattgg cgtttcctcc acctacgatg tttttgacca acagtttgct
      841 aatttcttta cttcgttctt tgctaccggt gaacagggta cccgcgtatt tggctacgta
      901 acgacaatgg gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat
      961 cgcatcg
//
