LOCUS       sequence-125252-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The RTRG repeat act as a nuclear/cytoplasmic partition signal in the
            expressed fusion protein providing increased nuclear to cytoplasmic 
            partition ratio when increasing the number of repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Christophe-Hobertus C, Christophe D
  TITLE     Identification of a short basic peptide motif able to drive 
            copy-number dependent nuclear accumulation of a linked protein.
  JOURNAL   Protein Pept Lett. 2008;15(4):397-401.
  PUBMED    18473954
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Pept Lett."; date: "2008"; volume: "15(4)"; pages: "397-401"
COMMENT     Sequence Label: pEGFP-(RTRG)3
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(13..117)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      144..599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(231..250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     441..462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(617..636)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
       61 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      121 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      301 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      481 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
      541 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
      601 cgcgttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaa
//
