LOCUS       sequence-125254-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OT-I TCR a chain cDNA in pCR1000.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Hogquist KA, Jameson SC, Heath WR, Howard JL, Bevan MJ, Carbone FR
  TITLE     T cell receptor antagonist peptides induce positive selection.
  JOURNAL   Cell. 1994 Jan 14;76(1):17-27.
  PUBMED    8287475
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1994-01-14"; volume: "76(1)"; pages: "17-27"
COMMENT     Sequence Label: p42.3A7
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(203..208,209..226,227..233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    241..257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     246..268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     265..281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     265..281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aggatctcaa gaagatcctt tgatcttttc tactgaacgg tgatccccac cggaattgcg
       61 gccgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      121 gcgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      241 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      301 atacgactca ctatagggcc cggtaccgag ctcactagtt taattaaaag cttatcggcc
      361 gaggtgagaa gggttcagat ctcaactgga ccacagcctc agcgtcatga gcaggttaaa
      421 tccggctact ttcagcagga ggattcggag tcccataact gacaggtttt gaaagtttag
      481 gttcatatct gtttcaaagc ttttctcagt caacgtggca tcacagggaa cgtctgaact
      541 ggggtaggtg gcgttggtct ctttgaagat atcttggcag gtgaagcttg tctggttgct
      601 ccaggcaatg gccccattgc tcttggaatc catagctttc atgtccagca cagttttgtc
      661 agtgatgaac gttccagatt ccatggtttt cggcacattg atttgggagt caaagtcggt
      721 gaacaggcag agggtgctgt cctgagaccg aggatctttt aactggtaca cagcaggttc
      781 tgggttctgg atgtctggct ttataattag cttggtccca gagccccaga tcaactgata
      841 gttgtcactt gctgcacaga agtaggtagc tgagtctcca ggctgagagt ctgtgatgtg
      901 caaggagagc tttttctccc ttttattgaa gaagattgtg aatcgtccat cttccttttt
      961 atcggacact gaacgtatgg atatcaggag tgcagggcct tccccaggga actgctggta
     1021 ccatgggaag tagttaaaag tgctgtcctc ataactgcag ttcagaattg cgg
//
