LOCUS       sequence-125255-        3754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OT-I TCR a chain cDNA in pCR1000.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Hogquist KA, Jameson SC, Heath WR, Howard JL, Bevan MJ, Carbone FR
  TITLE     T cell receptor antagonist peptides induce positive selection.
  JOURNAL   Cell. 1994 Jan 14;76(1):17-27.
  PUBMED    8287475
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1994-01-14"; volume: "76(1)"; pages: "17-27"
COMMENT     Sequence Label: p42.3A7
FEATURES             Location/Qualifiers
     source          1..3754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..84)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(238..826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(318..337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    983..1004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1019..1024,1025..1042,1043..1049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1057..1073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1062..1084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1081..1097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1081..1097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2064..2081)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2064..2080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2073..2095)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2403..3197)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SRLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVIHGDACLPNIMVENGRFSGFNDCGRLGVADRYQDIA
                     LDTRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     3124..3143
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(3665..3686)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
       61 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      421 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      661 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      841 cttttctact gaacggtgat ccccaccgga attgcggccg cccaatacgc aaaccgcctc
      901 tccccgcgcg ttggccgatt cattaatgca gctggcgcga caggtttccc gactggaaag
      961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     1021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     1081 caggaaacag ctatgaccat gattacgcca agctcaatac gactcactat agggcccggt
     1141 accgagctca ctagtttaat taaaagctta tcggccgagg tgagaagggt tcagatctca
     1201 actggaccac agcctcagcg tcatgagcag gttaaatccg gctactttca gcaggaggat
     1261 tcggagtccc ataactgaca ggttttgaaa gtttaggttc atatctgttt caaagctttt
     1321 ctcagtcaac gtggcatcac agggaacgtc tgaactgggg taggtggcgt tggtctcttt
     1381 gaagatatct tggcaggtga agcttgtctg gttgctccag gcaatggccc cattgctctt
     1441 ggaatccata gctttcatgt ccagcacagt tttgtcagtg atgaacgttc cagattccat
     1501 ggttttcggc acattgattt gggagtcaaa gtcggtgaac aggcagaggg tgctgtcctg
     1561 agaccgagga tcttttaact ggtacacagc aggttctggg ttctggatgt ctggctttat
     1621 aattagcttg gtcccagagc cccagatcaa ctgatagttg tcacttgctg cacagaagta
     1681 ggtagctgag tctccaggct gagagtctgt gatgtgcaag gagagctttt tctccctttt
     1741 attgaagaag attgtgaatc gtccatcttc ctttttatcg gacactgaac gtatggatat
     1801 caggagtgca gggccttccc cagggaactg ctggtaccat gggaagtagt taaaagtgct
     1861 gtcctcataa ctgcagttca gaattgcggt ctctccttcc cagactgtca gagattgggg
     1921 actttgtctc acctgctgct ggtcacgttt ctcctgctgc tggccattca ccccagctag
     1981 gtgaaggcct aggagtaaaa acgttgctgt cagaatcttg tccattcctg gatccaaacg
     2041 gttctcaccg cggccgcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2101 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2161 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     2221 cggccgcccg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     2281 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     2341 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     2401 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     2461 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     2521 cgggtatcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgttg
     2581 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatggatc
     2641 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     2701 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     2761 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     2821 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     2881 cgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     2941 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     3001 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     3061 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     3121 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     3181 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
     3241 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
     3301 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc
     3361 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg
     3421 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
     3481 ggactggctt tctacgtgtt ccggttcctt tagcagccct tgcgccctga gtgcttgcgg
     3541 cagcgtgaag ctaattcatg gttaaatttt tgttaaatca gctcattttt taaccaatag
     3601 gccgaaatcg caaaaatccc ttaataaatc aaaagaatag cccgagatag ggttgagtgt
     3661 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3721 aaaaaccgtc tatcagggcg atggcggatc agct
//
