LOCUS       sequence-125258-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human TACC3 
            (682-688aa ch-TOG binding site deleted) tagged with EGFP along with 
            shRNA to knock down endogenous TACC3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP∆682-688-shTACC3
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        45..259
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     180..199
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(332..348)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(333..349)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(377..395)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(416..432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(416..432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(429..451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    440..456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(464..470,471..488,489..494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    509..530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgaacgc tgacgtcatc
       61 aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc
      121 gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt
      181 cgctatgtgt tctgggaaat caccataaac gtgaaatgtc tttggatttg ggaatcttat
      241 aagttctgta tgagaccaca gatccccgtt accggaagat cgtctgttca agagacagac
      301 gatcttccgg taactttttg gaaaagctta tcgataccgt cgacctcgag ggggggcccg
      361 gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
      421 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      481 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      541 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gatagtaatc aattacgggg
      601 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      661 cctggctgac cgcccaacga cccccgcc
//
