LOCUS       sequence-125266-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-E2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(42..73)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcgatgagtg gcagggcggg gcgtaaacgc catgggcatg tagtcaaaag cctccggtcg
       61 gaggcttttg acttggctga ggagtgccgt taattaagtt taaatggggc ggatactttc
      121 ttcatccagc tccgcatcga agaaccgacg cagggtctga atattattat ccgcaataat
      181 ggactcgcgc gcctgcgcaa gtttttcttc gtatatcgcc tgacgccact ccagcggcgt
      241 acgcaccgcg ggattatcat cttcaacgat agtcagttca accgttgaac cttttaacat
      301 gctcaacgct tcagccagtt tttgctgtgc accgcggttg ttcaaatgcc gctgagagga
      361 gcgcaaatgc agacatactg cgttgtcgct ctcctctttc caggcattta acgccacctg
      421 ttcgaccagt tttggtagcg aaagttggct cacctgtgcc gcccacgggt cgcgctcaat
      481 ggcttctgcc gctagcttcg ccgccagttc cggcgttttt tcatgttcca gcgctttttt
      541 cagcgccttc ggcgtggcga ccacttcttt ttgctgcatc accggagtgg tcgccttcca
      601 gcgatacgct tcttttttgg ctggcgcttt ttccagcgcc gatggcaccg gacgcgcctg
      661 aacgcgatcg gtgaccgaag ccagtctttc cagcgcagcg ttattcaccg gccgcgcgcg
      721 ggtagcggct gccggttcac tcttttttgc tttggttgct ccctgcacgc gctgcaactg
      781 ctggcgcgcc gcc
//
