LOCUS       sequence-125268-         327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CYP2C8 cDNA IN PCw ORI+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 327)
  TITLE     Goldstein CYP2C9 and CYP2C9 in pOri+ for bacterial expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CYP2C8
FEATURES             Location/Qualifiers
     source          1..327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(30..35,36..51,52..58)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    66..82
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(125..130,131..146,147..153)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 catcgatgag cttactcccc atccccctgt tgacaattaa tcatcggctc gtataatgtg
       61 tggaattgtg agcggataac aatttcacac aggaaacagg atcagcttac tccccatccc
      121 cctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acaggatcca tcgatgctta ggaggtcata tggctctgtt attagcagtt
      241 tttctgtgtc tctcttttat gcttctcttt tcactctgga gacagagctg taggagaagg
      301 aagctccctc ctggccccac tcctctt
//
