LOCUS       sequence-125271-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-F3
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(41..72)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 cgatgagtgg cagggcgggg cgtaaacgcc atgggcatgt agtcaaaagc ctccggtcgg
       61 aggcttttga cttggctgag gagtgccgtt aattaagttt acaccaggct cttcaagcga
      121 taaatccact ccagcgcctg acgcggggtg agtgaatccg gatcaagatt ttccagagct
      181 tcgaccgcag gcgaagtttc ttctggtact gacagcaaag acatttgcgt accatccact
      241 tgcgtagcgg cggcgttcgg cgaaatgctt tccagctcac gcagcttttg ccgtgcgcgc
      301 ttaataacct cttttggcac gcctgccaga gctgcaaccg ccaggccgta gcttttgctc
      361 gccgcgccat cctgcacgct gtgcataaag gcaatggtgt cgccgtgctc cagtgcatcg
      421 agatgcacgt tagcgacgcc ttccattttc tccggtaact gggtcagctc gaaatagtgg
      481 gtagcaaata acgtcaatgc cttaatctta ttcgccagat tttccgcgca cgcccacgcc
      541 agcgacagac catcgtaggt ggacgttcca cgcccgatct gatccattaa caccagactg
      601 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
      661 cgcccggacg ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
      721 atctcgactt
//
