LOCUS       sequence-125283-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-J
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      733..764
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
       61 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
      121 cgtttttttg gacgcgtaca actcaagtct gacataaatg accgctatgg caagatattt
      181 gggtcctaag ctcaagctga gccgtcgtga gggcaccgac ttattcctta agtctggcgt
      241 tcgcgcgatc gataccaagt gtaaaattga acaagctcct ggccagcacg gtgcgcgtaa
      301 accgcgtctg tctgactatg gtgtgcagtt gcgtgaaaag caaaaagttc gccgtatcta
      361 tggtgtgctg gagcgtcagt tccgtaacta ctacaaagaa gcagcacgtc tgaaaggcaa
      421 caccggtgaa aacctgttgg ctctgctgga aggtcgtctg gacaacgttg tataccgtat
      481 gggcttcggt gccactcgtg cagaagcacg tcagctggtt agccataaag caattatggt
      541 aaacggtcgt gttgttaaca tcgcttctta tcaggttagt ccgaatgacg ttgtaagcat
      601 tcgtgagaaa gcgaagaagc agtctcgcgt gaaagccgct ctggagctgg ctgagcagcg
      661 tgaaaagcca acctggctgg aagttgatgc tggaaggtac gtttaaactt aattaacggc
      721 actcctcagc caagtcaaaa gcctccgacc ggaggctttt gactacatgc ccatggcgtt
      781 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      841 tggaagccat cacaaacggc atgatgaacc tga
//
