LOCUS       sequence-125290-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-K10
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc
       61 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac
      121 ccgttttttt ggacgcgtac aactcaagtc tgacataaat gaccgctatg agcactgcaa
      181 ttacacgcca gatcgttctc gctaccgcaa ccaccggtat gaaccagatt ggtgcgcact
      241 atgaaggcca caagatcatt gagattggtg ccgttgaagt ggtgaaccgt cgcctgacgg
      301 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
      361 tacatggtat tgccgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
      421 agttcatgga ctatattcgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatcg
      481 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aatactttct
      541 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaag cgcaacagcc
      601 tcgatgcgtt atgtgctcgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
      661 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
      721 tggcttttgc gatggaagga gagacacaac agcaacaagg tgaagcaaca attcagcgca
      781 ttgtacgtca ggcaagtaag ttacgcgttg tt
//
