LOCUS       sequence-125302-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD pduP1-18-gfpmut2-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: EYK054
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..764)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(54..764)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(94..114)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     709..737
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             829..840
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcttgcatg cttaagctgc taaagcgtag ttttcgtcgt ttgctgcgtc gactttgtat
       61 agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg
      121 tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct
      181 ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga
      241 acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt
      301 ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga
      361 atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca
      421 ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa aaatatagtt
      481 ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgctg tttcatatga
      541 tctgggtatc tcgcaaagca ttgaagacca tacgcgaaag tagtgacaag tgttggccat
      601 ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc gtatgttgca
      661 tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca
      721 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tacttctaga taattgctcg
      781 ctaagaatgg tgcgaatcag ggtttcgagt tcagaagtat tcatgacctt tctcctcttt
//
