LOCUS       sequence-125305-       10737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AacC2c1 full locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ001 (AacC2c1 locus)
FEATURES             Location/Qualifiers
     source          1..10737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8073..9263
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(8425..8444)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(10297..10737,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttttaga gtgttctgac
     1441 ggctaaaggc cgtgcttatc atccatcgaa aacggcaacg cacatttatc agatgttcgc
     1501 aatgatggac ccataaaagg gggccgtgag tgtctaagcc ggcagtacag cctattaggc
     1561 acaataatgc acattgttgg gtgaaaactc caagttcgtg gtggaaagga ggtttctgga
     1621 gtgaaaaagc cgaatctgaa aaatcagcaa aagcgcatca ccatcgcgct caggcttacg
     1681 gttgcatcgt cgcgacacaa gtaacaaacc cgtgtggatg gagtgcgcta ggtgcgctcc
     1741 atccccccat ccttgaacag aggtgcgaac atggccgtca aatccatcaa agtgaaactt
     1801 cgtctcgacg atatgccgga gattcgggcc ggtctatgga aacttcataa ggaagtcaat
     1861 gcgggggttc gatattacac ggaatggctc agtcttctcc gtcaagagaa cttgtatcga
     1921 agaagtccga atggggacgg agagcaagaa tgtgataaga ctgcagaaga atgcaaagcc
     1981 gaattgttgg agcggctgcg cgcgcgtcaa gtggagaatg gacaccgtgg tccggcggga
     2041 tcggacgatg aattgctgca gttggcgcgt caactctatg agttgttggt tccgcaggcg
     2101 ataggtgcga aaggcgacgc gcagcaaatt gcccgcaaat ttttgagccc cttggccgac
     2161 aaggacgcag ttggtgggct tggaatcgcg aaggcgggga acaaaccgcg gtgggttcgc
     2221 atgcgcgaag cgggggaacc aggctgggaa gaggagaagg agaaggctga gacgaggaaa
     2281 tctgcggatc ggactgcgga tgttttgcgc gcgctcgcgg attttgggtt aaagccactg
     2341 atgcgcgtat acaccgattc tgagatgtca tcggtggagt ggaaaccgct tcggaaggga
     2401 caagccgttc ggacgtggga tagggacatg ttccaacaag ctatcgaacg gatgatgtcg
     2461 tgggagtcgt ggaatcagcg cgttgggcaa gagtacgcga aactcgtaga acaaaaaaat
     2521 cgatttgagc agaagaattt cgtcggccag gaacatctgg tccatctcgt caatcagttg
     2581 caacaagata tgaaagaagc atcgcccgga ctcgaatcga aagagcaaac cgcgcactat
     2641 gtgacgggac gggcattgcg cggatcggac aaggtatttg agaagtgggg gaaactcgcc
     2701 cccgatgcac ctttcgattt gtacgacgcc gaaatcaaga atgtgcagag acgtaacacg
     2761 agacgattcg gatcacatga cttgttcgca aaattggcag agccagagta tcaggccctg
     2821 tggcgcgaag atgcttcgtt tctcacgcgt tacgcggtgt acaacagcat ccttcgcaaa
     2881 ctgaatcacg ccaaaatgtt cgcgacgttt actttgccgg atgcaacggc gcacccgatt
     2941 tggactcgct tcgataaatt gggtgggaat ttgcaccagt acaccttttt gttcaacgaa
     3001 tttggagaac gcaggcacgc gattcgtttt cacaagctat tgaaagtcga gaatggtgtc
     3061 gcaagagaag ttgatgatgt caccgtgccc atttcaatgt cagagcaatt ggataatctg
     3121 cttcccagag atcccaatga accgattgcg ctatattttc gagattacgg agccgaacag
     3181 catttcacag gtgaatttgg tggcgcgaag atccagtgcc gccgggatca gctggctcat
     3241 atgcaccgac gcagaggggc gagggatgtt tatctcaatg tcagcgtacg tgtgcagagt
     3301 cagtctgagg cgcggggaga acgtcgcccg ccgtatgcgg cagtatttcg tctggtcggg
     3361 gacaaccatc gcgcgtttgt ccatttcgat aaactatcgg attatcttgc ggaacatccg
     3421 gatgatggga agctcgggtc ggaggggttg ctttccgggc tgcgggtgat gagtgtcgat
     3481 ctcggccttc gcacatctgc atcgatttcc gtttttcgcg ttgcccggaa ggacgagttg
     3541 aagccgaact caaaaggtcg tgtaccgttt ttctttccga taaaagggaa tgacaatctc
     3601 gtcgcggttc atgagcgatc acaactcttg aagctgcctg gcgaaacgga gtcgaaggac
     3661 ctgcgtgcta tccgagaaga acgccaacgg acattgcggc agttgcggac gcaactggcg
     3721 tatttgcggc tgctcgtgcg gtgtgggtcg gaagatgtgg ggcggcgtga acggagttgg
     3781 gcaaagctta tcgagcagcc ggtggatgcg gccaatcaca tgacaccgga ttggcgcgag
     3841 gcttttgaaa acgaacttca gaagcttaag tcactccatg gtatctgtag cgacaaggaa
     3901 tggatggatg ctgtctacga gagcgttcgc cgcgtgtggc gtcacatggg caaacaggtt
     3961 cgcgattggc gaaaggacgt acgaagcgga gagcggccca agattcgcgg ctatgcgaaa
     4021 gacgtggtcg gtggaaactc gattgagcaa atcgagtatc tggaacgtca gtacaagttc
     4081 ctcaagagtt ggagcttctt tggtaaggtg tcgggacaag tgattcgtgc ggagaaggga
     4141 tctcgttttg cgatcacgct gcgcgaacac attgatcacg cgaaggaaga tcggctgaag
     4201 aaattggcgg atcgcatcat tatggaggct ctcggctatg tgtacgcgtt ggatgagcgc
     4261 ggcaaaggaa agtgggttgc gaagtatccg ccgtgccagc tcatcctgct ggaggaattg
     4321 agcgagtacc agttcaataa cgacaggcct ccgagcgaaa acaaccagtt gatgcaatgg
     4381 agtcatcgcg gcgtgttcca ggagttgata aatcaggccc aagtccatga tttactcgtt
     4441 gggacgatgt atgcagcgtt ctcgtcgcga ttcgacgcgc gaactggggc accgggtatc
     4501 cgctgtcgcc gggttccggc gcgttgcacc caggagcaca atccagaacc atttccttgg
     4561 tggctgaaca agtttgtggt ggaacatacg ttggatgctt gtcccctacg cgcagacgac
     4621 ctcatcccaa cgggtgaagg agagattttt gtctcgccgt tcagcgcgga ggagggggac
     4681 tttcatcaga ttcacgccga cctgaatgcg gcgcaaaatc tgcagcagcg actctggtct
     4741 gattttgata tcagtcaaat tcggttgcgg tgtgattggg gtgaagtgga cggtgaactc
     4801 gttctgatcc caaggcttac aggaaaacga acggcggatt catatagcaa caaggtgttt
     4861 tataccaata caggtgtcac ctattatgag cgagagcggg ggaagaagcg gagaaaggtt
     4921 ttcgcgcaag agaaattgtc ggaggaagag gcggagttgc tcgtggaagc agacgaggcg
     4981 agggagaaat cggtcgtttt gatgcgtgat ccgtctggca tcatcaatcg gggaaattgg
     5041 accaggcaaa aggaattttg gtcgatggtg aaccagcgga tcgaaggata cttggtcaag
     5101 cagattcgct cgcgcgttcc attacaagat agtgcgtgtg aaaacacggg ggatatttaa
     5161 cggcaaatgg gatttctcgc aatttcggac gagcttgcgg tgtttagaag tctctcaatc
     5221 ggctcagact ggtcatagat tgacagcgcg gttatggcac cagactgtag gtggagtgag
     5281 ggacatggcc gtaccgattc ggatgctgaa tgaactctct tattgcgaac gtttatatca
     5341 tttgatcatg tgcaagggtt attcgaaaaa tctgcggaca cagtggaagg cagcggtcag
     5401 cacgaccggg cggaacgccg caaaagacca tcagacgtgg ggcatgatgt gtgggatatt
     5461 gcaccgcaga gcctccatct cggcgacgag gatttgggga ttgtgggcaa attggatgcg
     5521 attcgctatc aggacgacgg gtattgggag cccgttgaag cgaaacattc aagcgcacca
     5581 aacggtggca cttcatttcg cctcggcgag tgggaattgg acggcaaggc gtggccgaat
     5641 gaccagatac agttgtgtgc acaaggattg ttactgagag ccaacggatt tccgaccatt
     5701 gcgggtaagt tgttctaccg aggaaacaga cagacggtaa ggattgtatt ttcggatgat
     5761 ttgattgctg caacggttgc caccatccgg agagcacacg agttggaaca ggcttcgatg
     5821 ccctcgccac tgctgaattc cgacaagtgc ttccgatgct cgttgaatgg tgtctgtctt
     5881 cctgacgaaa cgattcgctt gacgagtccg gacggagaga ccgttccgat acgagatatt
     5941 gtccccagtc gagatgattt aggaaccgtg tacgtgtcgg agcctgggac ccgggtcgga
     6001 aagcgtggtt acgagttgac catcacgtcg aaagatggga tggaatcgac cgttccgtta
     6061 aaagatgttc ggcacatctc cctcttcggg aacgttcaga tttccaccca attggtgcac
     6121 gattgtatgg agtccggcgt caccatcagc tacttgactg ccgccggccg tctcgtcggg
     6181 atgaaccaca atttgatttc gaaaaacgtc ttggtgcggc gtgaacagtt caagcggttt
     6241 ggggacgagc gtgatcgact gacacttgcg aggtatatcg ttcgcgcgaa aattctcaac
     6301 cagcgcacct tgcttcgtcg aaatgcgcac gggctcgaca aggtcatttt gaaagatttg
     6361 ctcgatcacg cccgacgttc cgaagaagcc acttccattg actcgttgct cggcattgaa
     6421 gggattgcgg cgaaacagta catgcaggcg tttgtgctga tgctgaaagt aaaaggcgtt
     6481 cctgcgggcg aaacgctgat gaacggcaga aatcgccgcc caccgaagga cccggttaat
     6541 gcgttgctgt cacttggata ttcgttgttg caacgggata tgtatgccgc cttggctgca
     6601 gtgggcctgg accccttgat gggcttttac catcgcattg aaacggggcg tccgtcactg
     6661 gtactcgaca tgatggaacc gtttcgagcg atcatcgcgg attcggtggt cgttcgaaca
     6721 ttgaacaccg gcgaaattgc ctgggatgac ttttatatcg ggcaggagtc ctgttcgttg
     6781 aagctgcctg gacggaaacg attttttcag gcgtttgagc gacgcatgca cgagaccgtg
     6841 acgcatccgg tgttcggtta caaattgagt taccgtcgga tgctggaact agaagcgcgc
     6901 ttcttgtcgc gatacttgat gggcgaattg ccggaatatc gcccaattgt cacgaggtga
     6961 tggcgatgcg ccgatatgtg ctggtgagct acgatatcag tgatcaaaaa cgctggcgaa
     7021 gggtatacaa aatcgttcga ggctatggca tgcacgttca gtattcggtg ttcctatgcc
     7081 aactcaccga tcaggatgag gccgagttga agcaattgct attggatgtg attcatcagt
     7141 cgatggatca agtcattttt gcaaggattg gaaccgtgca ggcgaaagct gccgagcgga
     7201 atatgtcgtg cattgggcgg gacttcgtac cattggattt gaagggattg gtattttgaa
     7261 ggattgatgt aaacttcaaa tacgcgtaaa atataatctg tctagaggac agaatttttc
     7321 aacgggtgtg ccaatggcca ctttccaggt ggcaaagccc gttgagcttc tcaaaaagaa
     7381 cgctcgctca gtgttctgac ctttcgagcg cctgttcagg gcgaaaaccc tgggaggcgc
     7441 tcgaatcata ggtgggacaa gggattcgcg gcgaaaacga cgggagagaa cgagaatgaa
     7501 acgagatgaa atcgatggga aacatcaagt gctcgtttat gggcttggat ctccgcgtgg
     7561 ttcaagggct gcgagcgccc gctgtcggat cactgagcga gcgatctgag aagtggcact
     7621 gtttggtaaa ggtaaaaaga cgaatgatgc atccgtcgga tcactgagcg agcgatctga
     7681 gaagtggcac cctttataaa aaggggcgtc ctttagtacc gtgtactgtc ggatcactga
     7741 gcgagcgatc tgagaagtgg cacaagcctt gagtaattcg ccgtgggatt ccccgccgta
     7801 tgtcggatca ctgagcgagc gatctgagaa gtggcactaa tgaagttaaa ggagatgaga
     7861 caatgaaaga agtcggatca ctgagcgagc gatctgagaa gtggcactgc aatgcgttgg
     7921 attatgacga tgcaggccaa ggaagtcgga tcactgagcg agcgatctga gaagtggcac
     7981 tgatgtcagc gaaacgacca ccatcggggt tataacagct ttaatgcggt agtttatcac
     8041 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     8101 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     8161 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     8221 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     8281 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     8341 tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
     8401 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     8461 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     8521 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     8581 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     8641 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     8701 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     8761 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     8821 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     8881 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     8941 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     9001 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     9061 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     9121 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     9181 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     9241 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     9301 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     9361 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     9421 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     9481 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     9541 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     9601 tgtttcgtaa agtctggaaa cgcggaagtc ccctacgtgc tgctgaagtt gcccgcaaca
     9661 gagagtggaa ccaaccggtg ataccacgat actatgactg agagtcaacg ccatgagcgg
     9721 cctcatttct tattctgagt tacaacagtc cgcaccgctg tccggtagct ccttccggtg
     9781 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     9841 aggacaggtg ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     9901 gccgaaacaa gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
     9961 accctgtgga acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg
    10021 gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac
    10081 tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
    10141 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
    10201 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc
    10261 aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc
    10321 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg
    10381 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
    10441 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
    10501 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
    10561 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
    10621 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
    10681 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacg
//
