LOCUS       sequence-125314-       10606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LshC2c2 full locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ003 (LshC2c2 locus)
FEATURES             Location/Qualifiers
     source          1..10606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3376..7545)
                     /codon_start=1
                     /product="type VI CRISPR effector from Leptotrichia shahii 
                     with RNA-guided RNAse activity (Abudayyeh et al., 2016)"
                     /label=LshC2c2
                     /translation="MGNLFGHKRWYEVRDKKDFKIKRKVKVKRNYDGNKYILNINENNN
                     KEKIDNNKFIRKYINYKKNDNILKEFTRKFHAGNILFKLKGKEGIIRIENNDDFLETEE
                     VVLYIEAYGKSEKLKALGITKKKIIDEAIRQGITKDDKKIEIKRQENEEEIEIDIRDEY
                     TNKTLNDCSIILRIIENDELETKKSIYEIFKNINMSLYKIIEKIIENETEKVFENRYYE
                     EHLREKLLKDDKIDVILTNFMEIREKIKSNLEILGFVKFYLNVGGDKKKSKNKKMLVEK
                     ILNINVDLTVEDIADFVIKELEFWNITKRIEKVKKVNNEFLEKRRNRTYIKSYVLLDKH
                     EKFKIERENKKDKIVKFFVENIKNNSIKEKIEKILAEFKIDELIKKLEKELKKGNCDTE
                     IFGIFKKHYKVNFDSKKFSKKSDEEKELYKIIYRYLKGRIEKILVNEQKVRLKKMEKIE
                     IEKILNESILSEKILKRVKQYTLEHIMYLGKLRHNDIDMTTVNTDDFSRLHAKEELDLE
                     LITFFASTNMELNKIFSRENINNDENIDFFGGDREKNYVLDKKILNSKIKIIRDLDFID
                     NKNNITNNFIRKFTKIGTNERNRILHAISKERDLQGTQDDYNKVINIIQNLKISDEEVS
                     KALNLDVVFKDKKNIITKINDIKISEENNNDIKYLPSFSKVLPEILNLYRNNPKNEPFD
                     TIETEKIVLNALIYVNKELYKKLILEDDLEENESKNIFLQELKKTLGNIDEIDENIIEN
                     YYKNAQISASKGNNKAIKKYQKKVIECYIGYLRKNYEELFDFSDFKMNIQEIKKQIKDI
                     NDNKTYERITVKTSDKTIVINDDFEYIISIFALLNSNAVINKIRNRFFATSVWLNTSEY
                     QNIIDILDEIMQLNTLRNECITENWNLNLEEFIQKMKEIEKDFDDFKIQTKKEIFNNYY
                     EDIKNNILTEFKDDINGCDVLEKKLEKIVIFDDETKFEIDKKSNILQDEQRKLSNINKK
                     DLKKKVDQYIKDKDQEIKSKILCRIIFNSDFLKKYKKEIDNLIEDMESENENKFQEIYY
                     PKERKNELYIYKKNLFLNIGNPNFDKIYGLISNDIKMADAKFLFNIDGKNIRKNKISEI
                     DAILKNLNDKLNGYSKEYKEKYIKKLKENDDFFAKNIQNKNYKSFEKDYNRVSEYKKIR
                     DLVEFNYLNKIESYLIDINWKLAIQMARFERDMHYIVNGLRELGIIKLSGYNTGISRAY
                     PKRNGSDGFYTTTAYYKFFDEESYKKFEKICYGFGIDLSENSEINKPENESIRNYISHF
                     YIVRNPFADYSIAEQIDRVSNLLSYSTRYNNSTYASVFEVFKKDVNLDYDELKKKFKLI
                     GNNDILERLMKPKKVSVLELESYNSDYIKNLIIELLTKIENTNDTL"
     CDS             7942..9132
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(8294..8313)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(10166..10606,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttaaatt aggctctagt
     1441 tagcctaatc gcataattat ttattatagt ataattctta ttttttttca acctaaaaat
     1501 ttaaaacatc tccaaaaatt ttcgtttcag aacaaccaag caaccatatt caaaaaacaa
     1561 taaaaaatga gcaagaattg aaattttatt ctcactcaga agttattttt attaaatatc
     1621 acttttcgat attggggtgg tctatatcaa tttaaaagac agaatagata attctttaga
     1681 gttttagtcc ccttcgatat tggggtggtc tatatcgaaa aagaagagtt tattcagata
     1741 gatttgtcgt tttagtcccc ttcgatattg gggtggtcta tatcctcatt tttacctttt
     1801 cccctttagt tatcagtttt agtccccttt gatattgggg tggtctatat caatatggat
     1861 tacttggtag aacagcaatc tagttttagt ccccttcgat attggggtgg tctatatcaa
     1921 tatggattac ttggtagaac agcaatctag ttttagtccc cttcgatatt ggggtggtct
     1981 atatcccatc ctaatttctt gctgatgaga tatttatttc taatttttct attttgtctt
     2041 tattttcaat actttcaatc ctatttttct ctttattaat aatatagaac caccctatac
     2101 tattatacca tattttttga tttttcaaaa ttccaatatt ttgttttgtg aaattttttc
     2161 tcccattgtc acttctcctg caagtacctt cattttttga aactgatctt ctgtcaggat
     2221 aatggaacgg attgatgaat tttctggagc gagcattgat aactgttttt ctgccagttc
     2281 gattttttct tttgttttcg acctcattat atataccgat ttttgaagct gataatatcc
     2341 cttttctatc aattttttcc taaaagtcct atattcaaat ctctcaacat ctgtctgcat
     2401 aggaaaatca tacataagca gaccaaaata ctcaatactc atagtccatc acgctcaatg
     2461 tcggaattat cacttcttca tcttttacaa aataatttcg tatactatcc aaataatagt
     2521 ctaccgcttg gaaaaaatca tatttcttat tgttaaataa taccttctgc tgtgctacaa
     2581 gaagtatttt ttgccttatt tccttactta atttcacttc attcaaaata tccttgtaca
     2641 tataaacaag ataatccacc ataggacgaa aaacctctat tatatcatca gaaaaattat
     2701 aggcattaaa ctgtgactta tgatgtaatc ctaaacttgg atgaaatcct tttgctacaa
     2761 tctttgatga tattatagct cttaaaatca tatatccata attaagtgca gaattcactc
     2821 catcttcatc aaatctttta aaactattac tatacaattc ctgaaaatat atccttgaag
     2881 ctattgcttc ctgatgttct gcactcgcat catctttttt caagttttcc ttatatgttt
     2941 tcagtctttc aatggaaata tcactttttt caagatactc taacaatgct ctttgatttt
     3001 caatcttatt ctccactatc ctgctccaca atttttcctt tttctctttt tcccactcaa
     3061 tctgctcatt tattcgtaaa gtcacttgaa aatgattaaa taatcccagc gaatgaattt
     3121 caggctgatg tttctcgttg caaataataa tcggaatgtt attttccacc agcctcaact
     3181 gcaaaatcgc actaatctta caatagcagt tttcaataac tatcgcagat atatcattca
     3241 aagaaatctt atttttctca tcattattgt cttcatcaac cattataagc tgattattcg
     3301 atattgacaa atcatcagcc cttgttatgt gaattatatt gggcatttta atcatactcc
     3361 ttataaattt cattcttata acgtatcatt cgtattttct atttttgtta aaagttctat
     3421 tatcaagttt ttaatataat cagaattata actttctaat tctaaaacag aaactttttt
     3481 aggtttcatt aatctttcaa gtatatcatt attaccgata agtttaaatt ttttctttaa
     3541 ttcatcataa tctaaattca catctttttt aaatacttca aatacacttg cataagttga
     3601 attattataa cgtgtactat atgataataa attagaaact ctatcaattt gttctgcaat
     3661 actgtaatca gcaaacggat ttcttacaat atagaaatgt gaaatatagt ttctaatact
     3721 ttcattttcc ggcttattaa tttcagaatt ttcagacaaa tcaattccaa atccataaca
     3781 tattttctca aattttttat aagattcttc atcaaaaaat ttatagtatg ctgttgttgt
     3841 ataaaagcca tcagatccat tacgcttagg ataagctcta cttattccag tattgtagcc
     3901 acttaactta ataattccta attctcttag cccatttaca atatagtgca tatctctttc
     3961 aaatctagcc atttgaatag caagtttcca atttatatct atcaaataac tttctatttt
     4021 attcaaataa ttaaattcta ccaaatctct aatttttttg tattcagaaa ctctattata
     4081 atctttttca aatgatttat agtttttatt ttgtatattt tttgcaaaaa agtcatcatt
     4141 ttctttcaat ttttttatat acttctcttt gtattcttta gaatatccat ttagtttatc
     4201 atttagattt ttcaatattg catcaatttc agatatttta ttttttctaa tatttttacc
     4261 atcaatatta aataaaaatt ttgcatcagc cattttaata tcatttgaaa ttaatccata
     4321 aattttatca aaatttggat ttccaatatt taaaaataaa ttctttttat aaatatataa
     4381 ttcattctta cgttctttag gataatatat ttcttgaaat ttattttcat tctctgattc
     4441 catatcttct attaaattat ctatttcttt tttgtatttt tttaaaaaat cagaattaaa
     4501 tattattcta cacaatattt tactctttat ttcctgatct ttatctttta tatactgatc
     4561 aacctttttt ttcaaatcct ttttatttat gtttgataac tttctttgtt catcttgtaa
     4621 tatattcgat tttttatcta tctcaaattt agtttcatca tcaaaaatta caattttttc
     4681 taattttttc tctaaaacat cacaaccatt aatatcatct ttaaattcag ttaatatatt
     4741 attttttata tcctcataat aattattaaa aatttctttt ttagtttgta ttttaaaatc
     4801 atcaaagtct ttttctatct ctttcatttt ttgaataaat tcttctaaat taagattcca
     4861 attttcagtt atacattcat ttctcaaagt atttaattgc attatttcat ctaaaatatc
     4921 tataatattt tgatattctg aagtatttaa ccaaactgat gttgcaaaaa atctatttct
     4981 aattttattt ataaccgcat tactatttaa cagtgcaaat attgaaatta tatattcaaa
     5041 atcatcattt attactatag ttttatcact agtctttaca gttattcttt cgtaagtttt
     5101 attatcatta atgtctttta tttgtttctt aatttcttga atattcattt taaaatctga
     5161 aaaatcaaaa agttcctcat aattttttct caaatatcca atataacatt ctattacttt
     5221 tttctgatat tttttaatag ctttattatt accttttgaa gcagaaatct gagcattttt
     5281 ataataattt tctataatat tttcatctat ttcatcaatg tttcctaaag ttttctttaa
     5341 ttcttgtaaa aatatattct tactttcatt ttcttctaaa tcatcttcta aaattaattt
     5401 cttatacaat tctttattca catatattaa agcatttaat actatttttt ctgtttctat
     5461 agtatcaaat ggttcattct taggattatt cctatataaa tttaatattt caggaagtac
     5521 tttagaaaag gatggtaaat atttaatatc attattattt tcttctgaaa ttttaatatc
     5581 atttatttta gtaattatat tttttttatc tttaaatact acatctaaat ttaatgcttt
     5641 tgacacttct tcatctgata tttttaaatt ttgaattata tttatgactt tattatagtc
     5701 atcttgcgtt ccttgtaaat ctctttcctt gctaatcgca tgtaatatcc tgtttctttc
     5761 atttgttcct atctttgtaa atttcctaat aaaattattt gtaatgttat ttttattatc
     5821 tataaaatct aagtctctta ttatttttat ttttgaattt aaaatttttt tatcaagtac
     5881 gtaatttttt tctcgatctc ctccaaagaa atctatattt tcatcattat ttatattttc
     5941 tctagaaaaa atcttattta attccatatt ggtagaagca aaaaaagtaa tcaattctaa
     6001 atccaattcc tctttagcgt gaagtctaga aaaatcatca gtatttactg ttgtcatatc
     6061 tatatcatta tgtcttaatt tccctaaata cataatatgc tctaacgtat attgcttaac
     6121 tctttttaaa attttttcag ataatatact ttcatttaaa attttttcta tttctatttt
     6181 ttccattttc tttaatctga ctttttgttc atttaccaat attttttcaa ttcttccttt
     6241 caaatatcga tatatgattt tatatagttc tttttcttca tcagatttct ttgaaaattt
     6301 tttcgaatca aaattaactt tataatgttt tttaaatatt ccaaaaattt ctgtatcaca
     6361 atttcctttt tttagttctt tttctaattt ttttattaat tcatctattt taaattctgc
     6421 taaaattttt tctatttttt cttttatact attatttttt atattttcta caaaaaattt
     6481 tacaatttta tcttttttat tttctctttc tattttaaat ttttcgtgct tatctaatag
     6541 tacataagat tttatatatg ttctatttct tctcttttca agaaattcat tattaacttt
     6601 ttttactttt tcaattcttt tagtaatatt ccaaaattct aactctttta taacaaaatc
     6661 agctatatct tctactgtta aatctacatt tatatttaaa attttttcaa caagcatttt
     6721 tttattttta gatttctttt tatcaccacc aacattaaga taaaatttta caaaacccag
     6781 aatttctaaa ttacttttta ttttttctct tatttccata aaattagtca aaataacatc
     6841 tattttatca tctttcaata atttttctct taaatgttct tcataatatc gattttcaaa
     6901 tactttttct gtttcatttt caattatttt ttctataatc ttatataaac tcatgttaat
     6961 atttttaaaa atttcgtaaa ttgatttttt tgtttctaat tcatcatttt ctattattct
     7021 taatattatt gaacaatcat ttagtgtttt attagtatac tcatctctga tatctatctc
     7081 tatttcttct tcattctctt gtctctttat ttctattttt ttatcatctt tagttattcc
     7141 ttgcctaatt gcttcatcta ttattttctt ttttgtaatc cccaatgctt tcaatttctc
     7201 agattttcca tatgcttcta tatataatac aacttcttct gtttccaaaa aatcatcatt
     7261 attttctatt cttatgattc cttctttacc tttcaactta aatagaatat ttcctgcatg
     7321 aaattttctt gtaaattctt taagaatatt atcatttttt ttgtaattaa tatattttct
     7381 aataaattta ttattatcaa ttttttcttt attattattt tcattaatat ttaaaatgta
     7441 tttgtttcca tcatagttcc ttttaacttt tactttccgt tttattttaa aatctttttt
     7501 atcacgaact tcataccatc tcttatgtcc aaataaattt cccattccaa tctcctcgtt
     7561 tctactttaa tctaataaaa tatttttaaa ttaaatcaat tttacatctt tctaatcaaa
     7621 aatacaattt tccattttta gtataccaca tcaatattaa atctcaaaaa aataaggagc
     7681 cgtcaaacat agctccctac ttctatttac tcataatccc catctatcct tacttttcgt
     7741 aaaatcaatc cttctttcgc ctttagatcc aacttaattt tcccatttga acctgttcta
     7801 aatgttctgc cttctgttac caaatcaata aatctttcat cctgataatt tgtttcaaat
     7861 tccacatttt cccagctgtt aaacgagctt taatgcggta gtttatcaca gttaaattgc
     7921 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     7981 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     8041 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     8101 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     8161 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     8221 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     8281 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     8341 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     8401 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     8461 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     8521 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     8581 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     8641 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     8701 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     8761 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     8821 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     8881 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     8941 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     9001 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     9061 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     9121 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     9181 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     9241 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     9301 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     9361 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     9421 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     9481 gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac
     9541 caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt
     9601 attctgagtt acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca
     9661 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     9721 cggcagcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     9781 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     9841 cacctacatc tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata
     9901 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     9961 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
    10021 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
    10081 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag
    10141 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
    10201 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
    10261 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
    10321 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
    10381 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
    10441 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
    10501 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
    10561 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacg
//
