LOCUS       sequence-125321-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Gerard Cagney unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCR8/GW/TOPO GFP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             112..807
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     protein_bind    complement(821..845)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgaat tcgcccttac catggagagc
      121 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
      181 gtggagttcg agctggtggg cggcggagag ggcacccccg agcagggccg catgaccaac
      241 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
      301 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
      361 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
      421 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
      481 atgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
      541 gccaccgtgg agcacctgca ccccatgggc gataacgatc tggatggcag cttcacccgc
      601 accttcagcc tgcgcgacgg cggctactac agctccgtgg tggacagcca catgcacttc
      661 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
      721 gtggaggagg atcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
      781 accccggatg cagatgccgg tgaagaaaag ggcgaattcg acccagcttt cttgtacaaa
      841 gttggcatta taaaaaataa ttgctcatca atttgttgca acgaacaggt cactatcagt
      901 caaaataaaa tc
//
