LOCUS       sequence-125325-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD eutE1-20- gfpmut2-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ119
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(132..160)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     755..775
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggctagcga attcgagctc ttaaagagga gaaaggtcat gaatcaacag gatattgaac
       61 aggtggtgaa agcggtactg ctgaaaatga aagacagctc tagaagtaaa ggagaagaac
      121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      181 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcgcgtatg
      301 gtcttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
      361 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      421 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      481 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      541 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      601 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      721 tttcgaaaga tcccaacgaa aagagagatc acatggtcct tcttgagttt gtaacagctg
      781 ctgggattac acatggcatg
//
