LOCUS       sequence-125330-       10239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AacC2c1 human codon optimized protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, 
            Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, 
            Koonin EV
  TITLE     Discovery and Functional Characterization of Diverse Class 2 
            CRISPR-Cas Systems.
  JOURNAL   Mol Cell. 2015 Nov 5;60(3):385-97. doi: 
            10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
  PUBMED    26593719
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.008"; journalName: "Mol Cell"; date: 
            "2015-11-5- 5"; volume: "60"; issue: "3"; pages: "385-97"
COMMENT     Sequence Label: pZ005 (AacC2c1 human codon optimized protein)
FEATURES             Location/Qualifiers
     source          1..10239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2404..2546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2472..2491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2632..2654
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3648..4730)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4692..4711
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4731..4808)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5014..5033
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5117..5136
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5117..5135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5136..5160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5175..5197
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5211..5228
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      6183..6192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6345..6362
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6369..6389
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6396..6416
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      9228..9237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9840..9887
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9894..9974
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             9999..10022
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10023..10067
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             10083..10100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10153..10171)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10167..10214
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggttcacg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggt catcatcatc
     5221 accatcacaa aatcgaagaa ggcaaactgg ttatttggat caatggcgat aaaggctata
     5281 atggtctggc agaagttggc aaaaaattcg aaaaagatac cggcattaaa gtgaccgttg
     5341 aacatccgga taaactggaa gaaaaatttc cgcaggttgc agcaaccggt gatggtccgg
     5401 atattatctt ttgggcacat gatcgttttg gtggttatgc acagagcggt ctgctggcag
     5461 aaattacacc ggataaagca tttcaggaca aactgtatcc gtttacctgg gatgcagttc
     5521 gctataacgg taaactgatt gcatatccga ttgcagttga agcactgagc ctgatctata
     5581 acaaagatct gctgccgaat ccgcctaaaa cctgggaaga aattccggca ctggataaag
     5641 aactgaaagc aaaaggtaaa agcgcactga tgtttaatct gcaagaaccg tattttacct
     5701 ggcctctgat tgcagcagat ggtggctatg cattcaaata tgaaaacggc aaatatgata
     5761 tcaaagacgt gggtgttgat aatgcgggtg caaaagccgg tctgaccttt ctggttgatc
     5821 tgattaaaaa caaacacatg aacgccgata ccgattatag cattgcagaa gcagcattta
     5881 acaaaggtga aaccgcaatg acaattaatg gtccgtgggc atggtcaaat attgatacca
     5941 gcaaagtgaa ttatggtgtt accgttctgc cgacatttaa aggtcagccg agcaaaccgt
     6001 ttgttggtgt tctgagcgca ggtattaatg cagcaagccc gaacaaagaa ctggcaaaag
     6061 aatttctgga aaactatctg ctgaccgatg aaggtctgga agcagtgaat aaagataaac
     6121 cgctgggtgc agttgcactg aaaagctatg aagaagaact ggttaaagat ccgcgtattg
     6181 cagccaccat ggaaaatgca cagaaaggcg aaattatgcc gaatattccg cagatgagcg
     6241 cattttggta tgccgttcgt accgcagtga ttaatgccgc atcaggtcgt cagaccgttg
     6301 atgaagccct gaaagatgcc cagaccggtg caagcaccgg atccctggtt ccgcgtggta
     6361 gcgctagcga aaatctgtat tttcagggaa tggccccaaa gaagaagcgg aaggtcggta
     6421 tccacggagt cccagcagcc ggtagtgctg tgaagtctat caaagtgaag ctccgcctgg
     6481 atgacatgcc cgagataagg gcagggctgt ggaagctgca taaagaggtt aacgctggcg
     6541 tacggtacta caccgaatgg ctctcacttt tgcggcagga aaacctttac agaagatctc
     6601 ctaacggaga cggagaacaa gagtgcgaca agactgcgga ggaatgcaaa gcggagctgt
     6661 tggagaggct tagggcccgg caggtggaga atggccacag gggtcccgcg ggcagcgatg
     6721 atgagctgct gcagcttgct aggcagcttt atgagctgtt ggttccgcag gcaatcgggg
     6781 cgaaaggcga tgctcagcag atcgcccgaa aattcttgtc tccccttgct gacaaggacg
     6841 ctgttggggg tctgggtata gcgaaagctg gaaataagcc cagatgggtg agaatgcgcg
     6901 aagctggcga acccgggtgg gaagaagaaa aagagaaggc agagactagg aaatccgccg
     6961 atcggacagc agatgtactt cgcgcactcg ccgatttcgg gctcaaacca ctgatgcggg
     7021 tttacactga ctcagagatg tcctctgtcg aatggaaacc tttgcgcaaa ggccaggctg
     7081 ttaggacatg ggaccgggac atgttccagc aggcgatcga aaggatgatg tcatgggagt
     7141 cttggaacca gagagtcggg caggagtatg caaaactggt tgaacagaag aaccggttcg
     7201 aacaaaagaa ttttgtcggc caagagcatc tggtgcacct ggtaaaccaa ttgcagcagg
     7261 atatgaagga ggcctcccca ggtttggaga gcaaggaaca aaccgcccat tatgttactg
     7321 gccgagcact gcgaggttca gataaggtct ttgagaaatg gggcaagctg gcacccgatg
     7381 ccccttttga cctgtacgac gcagagatca aaaacgtcca gcgccgaaac acgcgcaggt
     7441 tcggctctca tgacctgttt gcaaaactgg ccgagcccga gtaccaggct ctgtggcggg
     7501 aagatgcaag ctttctgacc cgatacgccg tgtataattc catactgcga aaactcaacc
     7561 acgcaaaaat gtttgcaacc ttcaccctgc cagacgctac ggctcatccc atatggacca
     7621 gatttgataa actcggtggt aacctgcatc agtatacttt tctgttcaat gagttcggcg
     7681 agcgccgcca cgcaatccgc tttcataaac ttttgaaggt tgagaatggg gtggcccgcg
     7741 aggtggatga cgtgaccgta ccaatcagca tgtctgagca gctcgacaac ctgcttccta
     7801 gagatcctaa cgagccaatc gccctctatt ttagagatta tggcgcggag caacatttca
     7861 caggcgagtt cgggggggct aagatccaat gcaggaggga tcagcttgcc catatgcata
     7921 ggcgcagagg cgcaagagac gtctacctga atgtgtcagt gcgggtgcag agtcaaagtg
     7981 aagccagggg ggaacgccgg cctccgtatg ccgcggtttt ccgcctggtg ggggataatc
     8041 acagggcatt cgtccatttc gataagctct ccgattatct tgcagaacat ccagacgatg
     8101 gcaaattggg gtccgagggt ttgctgtcag gtctgagggt gatgtccgtg gatctgggcc
     8161 tgaggacctc cgccagtatc tcagttttta gagtggctcg caaggacgaa ctcaagccaa
     8221 acagtaaagg gcgcgttcca tttttctttc ccattaaggg caacgacaat cttgtggctg
     8281 ttcacgagag atcccagctg ttgaaactcc ccggcgagac agagtccaaa gatctcagag
     8341 ctattagaga agaacggcag aggacactga ggcagttgag gacccagctg gcctacttgc
     8401 gactgctggt tcgctgcggg tccgaagatg tgggacgccg cgaacgatcc tgggctaaac
     8461 tgatcgaaca gcccgttgac gccgctaacc atatgacccc agactggagg gaagcgttcg
     8521 agaacgagtt gcagaagctg aagagccttc atgggatctg cagtgataag gaatggatgg
     8581 acgcagttta cgagagtgta cgaagagttt ggcgacacat ggggaagcag gtgagagatt
     8641 ggaggaagga cgtcaggtca ggtgagagac ctaagatccg gggttacgca aaggacgttg
     8701 tgggaggtaa ctccattgaa cagatagaat acctggaaag gcagtataag ttcctgaaat
     8761 cctggtcctt tttcggcaag gttagtggac aggtgatccg ggcagaaaag gggtctagat
     8821 tcgcgattac cctgcgcgag catatcgatc acgccaaaga ggaccggctt aagaagcttg
     8881 ccgaccgaat aataatggag gctcttggtt acgtctatgc tctcgatgag cgaggcaaag
     8941 ggaaatgggt ggcgaaatac cctccctgcc agctcatcct gctggaagag ctgagcgagt
     9001 accagtttaa caacgatcga cccccaagcg agaataatca gctcatgcag tggtctcacc
     9061 gaggggtttt tcaagaactg atcaatcagg cacaagtgca tgacctcctt gtgggcacta
     9121 tgtacgcagc cttcagcagt agatttgatg cacgcaccgg tgctcctggc attcggtgtc
     9181 gacgggtgcc cgcaagatgt actcaagaac ataatcctga accgttccca tggtggctga
     9241 acaaattcgt tgtggaacat accctcgacg catgccccct gcgcgccgat gacctgattc
     9301 caaccggaga gggcgagata tttgtgagtc cttttagcgc cgaagaggga gactttcacc
     9361 agattcacgc ggacttgaat gccgctcaga acctgcagca gaggctctgg agtgatttcg
     9421 acatctctca gatccgcctg cgctgtgatt ggggcgaagt ggatggtgag ctggtgctga
     9481 ttccaaggtt gaccggcaag aggacagcag acagttattc taacaaggtg ttctatacca
     9541 ataccggcgt tacttattac gagcgggaaa ggggaaagaa gaggagaaag gtgttcgcgc
     9601 aggagaagct gagcgaggag gaagccgagc tcctcgtgga ggccgatgaa gcccgagaga
     9661 agagtgttgt tctgatgcgc gatccctctg ggatcattaa ccgaggcaat tggacccgcc
     9721 agaaagaatt ttggtccatg gtcaatcagc ggatcgaggg ctacttggtg aaacagataa
     9781 ggagtcgggt cccccttcag gacagcgcct gcgaaaatac aggagacatt ggcagcggga
     9841 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat
     9901 acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac ccatacgatg
     9961 tccccgacta tgcctaaagc ctcgagggtg gtggtagcga ttataaagat gatgatgata
    10021 aaggcctgaa cgatatcttt gaagcccaga aaattgaatg gcacgagtaa aagcttgtcg
    10081 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
    10141 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
    10201 tgaggggttt tttgctgaaa ggaggaacta tatccggat
//
