LOCUS       sequence-125334-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orthogonal GFP message under lpp5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS
  TITLE     Protein synthesis by ribosomes with tethered subunits.
  JOURNAL   Nature. 2015 Jul 29. doi: 10.1038/nature14862.
  PUBMED    26222032
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jul 29. doi: 10.1038/nature14862."
COMMENT     Sequence Label: pLpp5oGFP
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    160..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
       61 aacgtggctt tccccccccc ccctgcaggt cgacggatcc taatacgact cactatcaaa
      121 aaaatattga caacataaaa aactttgtgt tatacttgtg gaattgtgag cggataacaa
      181 ttctatatct gttatttttt cacaccacag atctatgagc aaaggtgaag aactgtttac
      241 cggcgttgtg ccgattctgg tggaactgga tggcgatgtg aacggtcaca aattcagcgt
      301 gcgtggtgaa ggtgaaggcg atgccacgat tggcaaactg acgctgaaat ttatctgcac
      361 caccggcaaa ctgccggtgc cgtggccgac gctggtgacc accctgacct atggcgttca
      421 gtgttttagt cgctatccgg atcacatgaa acgtcacgat ttctttaaat ctgcaatgcc
      481 ggaaggctat gtgcaggaac gtacgattag ctttaaagat gatggcaaat ataaaacgcg
      541 cgccgttgtg aaatttgaag gcgataccct ggtgaaccgc attgaactga aaggcacgga
      601 ttttaaagaa gatggcaata tcctgggcca taaactggaa tacaacttta atagccataa
      661 tgtttatatt acggcggata aacagaaaaa tggcatcaaa gcgaatttta ccgttcgcca
      721 taacgttgaa gatggcagtg tgcagctggc agatcattat cagcagaata ccccgattgg
      781 tgatggtccg gtgctgctg
//
