LOCUS       sequence-125335-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orthogonal GFP message under lpp5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS
  TITLE     Protein synthesis by ribosomes with tethered subunits.
  JOURNAL   Nature. 2015 Jul 29. doi: 10.1038/nature14862.
  PUBMED    26222032
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jul 29. doi: 10.1038/nature14862."
COMMENT     Sequence Label: pLpp5oGFP
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..70)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(71..118)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     protein_bind    798..821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atcgatcttc agcggccgcg ggctttgtta gcagccggtc gacttatttt tcgaactgcg
       61 gatggctcca cgtaatacct gccgcattca catattcgtg cagaaccatg tggtcccgcg
      121 tgcctttttc gttcggatct ttagacagaa cggtctgcgt gctcagataa tgattatccg
      181 gcagcagcac cggaccatca ccaatcgggg tattctgctg ataatgatct gccagctgca
      241 cactgccatc ttcaacgtta tggcgaacgg taaaattcgc tttgatgcca tttttctgtt
      301 tatccgccgt aatataaaca ttatggctat taaagttgta ttccagttta tggcccagga
      361 tattgccatc ttctttaaaa tccgtgcctt tcagttcaat gcggttcacc agggtatcgc
      421 cttcaaattt cacaacggcg cgcgttttat atttgccatc atctttaaag ctaatcgtac
      481 gttcctgcac atagccttcc ggcattgcag atttaaagaa atcgtgacgt ttcatgtgat
      541 ccggatagcg actaaaacac tgaacgccat aggtcagggt ggtcaccagc gtcggccacg
      601 gcaccggcag tttgccggtg gtgcagataa atttcagcgt cagtttgcca atcgtggcat
      661 cgccttcacc ttcaccacgc acgctgaatt tgtgaccgtt cacatcgcca tccagttcca
      721 ccagaatcgg cacaacgccg gtaaacagtt cttcaccttt gctcatagat ctgtggtgtg
      781 aaaaaataac agatatagaa ttgttatccg ctcacaattc cacaagtata acacaaagtt
      841 ttttatgttg tcaatatttt tttgatagtg agtcgtatta ggatc
//
