LOCUS       sequence-125338-         450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD etuAld+Ile1-21-gfpmut2- ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ137
FEATURES             Location/Qualifiers
     source          1..450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..18
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(119..147)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctcttaaag aggagaaagg tcatggataa ggacttacag tcaatacagg aaattgtaag
       61 aactcttata gcaaaagcaa agaaatctag aagtaaagga gaagaacttt tcactggagt
      121 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      181 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      241 aaaactacct gttccatggc caacacttgt cactactttc gcgtatggtc ttcaatgctt
      301 tgcgagatac ccanatcata tgaaacagca tgactttttc aagantgcca tgcccgaagg
      361 ttatgtacag gaaagaacta tatttttcan agatgacggg aactacaaga cacgtgctga
      421 agtcaagttt gaaggtgata cccttgttaa
//
