LOCUS       sequence-125340-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBAD etuAld+Ile1-21-gfpmut2- ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ137
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..758)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(48..758)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(88..108)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     703..731
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             832..843
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcttttaag ctgctaaagc gtagttttcg tcgtttgctg cgtcgacttt gtatagttca
       61 tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtgatctctc
      121 ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa
      181 aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt
      241 ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt ttgtttgtct
      301 gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt
      361 ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca
      421 aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc
      481 tgtacataac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat atgatctggg
      541 tatctcgcaa agcattgaag accatacgcg aaagtagtga caagtgttgg ccatggaaca
      601 ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct
      661 tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa ttcaacaaga
      721 attgggacaa ctccagtgaa aagttcttct cctttacttc tagatttctt tgcttttgct
      781 ataagagttc ttacaatttc ctgtattgac tgtaagtcct tatccatgac ctttctcctc
      841 tttaagagct
//
