LOCUS       sequence-125343-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTET eutE1-20-pduD21-224-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ157
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        64..119
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta attcctaatt
       61 tttgttgaca ctctatcgtt gatagagtta ttttaccact ccctatcagt gatagagaaa
      121 agagaattct taaagaggag aaaggtcatg aatcaacagg atattgaaca ggtggtgaaa
      181 gcggtactgc tgaaaatgaa agacagcggc agcgataaac ccgtctcgtt taatgcgcct
      241 gcggcatcca cagcaccaca gaccgctgcg cctgcgggcg acggctttct gaccgaagtg
      301 ggcgaagcgc gccagggcac tcagcaggac gaagtcatta tcgccgtcgg cccggcattt
      361 ggcctggcgc aaaccgtcaa tatcgtcggc ttaccgcata agagcattct gcgcgaagtc
      421 attgccggta ttgaagaaga aggcatcaag gcgcgcgtga ttcgctgctt taaatcttcc
      481 gacgtggcgt tcgtcgccgt tgaaggtaac cgcctgagcg gatccggcat ctccatcggc
      541 atccagtcga aaggtactac ggttatccac cagcaggggc taccgccgct ctccaacctg
      601 gagctgttcc cgcaggcacc gctgctgacg ctggaaacct accgtcagat tggtaaaaac
      661 gccgcccgct atgcgaaacg agaatcaccg cagccggtcc ctacgctcaa tgaccagatg
      721 gcacgcccga agtaccaggc aaagtcggcc attttgcata ttaaagagac caagtacgtc
      781 gtgacggg
//
