LOCUS       sequence-125345-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTET HochAld1-20-pduD21-224- FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ159
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..113
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    94..112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             126..137
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
       61 gacactctat cgttgataga gttattttac cactccctat cagtgataga gaaaagagaa
      121 ttcttaaaga ggagaaaggt catggctctg cgcgaggatc gcatcgccga gatcgtcgag
      181 cgcgtgctcg cgcgcctcga ctctggcagc gataaacccg tctcgtttaa tgcgcctgcg
      241 gcatccacag caccacagac cgctgcgcct gcgggcgacg gctttctgac cgaagtgggc
      301 gaagcgcgcc agggcactca ncaggacgaa gtcattatcg ccgtcggccc ggcatttggc
      361 ctggcgcaaa ccgtcaatat cgtcggctta ccgcataaga gcattctgcg cgaagtcatt
      421 gccggtattg aagaagaagg catcaaggcg cgcgtgattc gctgctttaa atcttccgac
      481 gtggcgttcg tcgccgttga aggtaaccgc ctgagcggat ccggcatctc catcggcatc
      541 cagtcgaaag gtactacggt tatccaccag caggggctac cgccgctctc caacctggag
      601 ctgttcccgc aggcaccgct gctgacgctg gaaacctacc gtcagattgg taaaaacgcc
      661 gcccgctatg cgaaacgaga atcaccgcag ccggtcccta cgctcaatga ccagatggca
      721 cgcccgaagt accaggcaaa
//
