LOCUS       sequence-125346-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJL1 expression vector with sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, 
            Majewska NI, Chen CX, Marcum RD, Weiss MG, Stoddart AE, Amiram M, 
            Ranji Charna AK, Patel JR, Isaacs FJ, Kelleher NL, Hong SH, Jewett 
            MC
  TITLE     Cell-free protein synthesis from genomically recoded bacteria 
            enables multisite incorporation of noncanonical amino acids.
  JOURNAL   Nat Commun. 2018 Mar 23;9(1):1203. doi: 10.1038/s41467-018-03469-5.
  PUBMED    29572528
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-23"; volume: "9(1):1203. doi"; pages: " 
            10.1038/s41467-018-03469-5"
COMMENT     Sequence Label: pJL1
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             639..686
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             687..710
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(765..783)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gtgaagaact gtttaccggc gttgtgccga ttctggtgga actggatggc gatgtgaacg
       61 gtcacaaatt cagcgtgcgt ggtgaaggtg aaggcgatgc cacgattggc aaactgacgc
      121 tgaaatttat ctgcaccacc ggcaaactgc cggtgccgtg gccgacgctg gtgaccaccc
      181 tgacctatgg cgttcagtgt tttagtcgct atccggatca catgaaacgt cacgatttct
      241 ttaaatctgc aatgccggaa ggctatgtgc aggaacgtac gattagcttt aaagatgatg
      301 gcaaatataa aacgcgcgcc gttgtgaaat ttgaaggcga taccctggtg aaccgcattg
      361 aactgaaagg cacggatttt aaagaagatg gcaatatcct gggccataaa ctggaataca
      421 actttaatag ccataatgtt tatattacgg cggataaaca gaaaaatggc atcaaagcga
      481 attttaccgt tcgccataac gttgaagatg gcagtgtgca gctggcagat cattatcagc
      541 agaatacccc gattggtgat ggtccggtgc tgctgccgga taatcattat ctgagcacgc
      601 agaccgttct gtctaaagat ccgaacgaaa aaggcacgcg ggaccacatg gttctgcacg
      661 aatatgtgaa tgcggcaggt attacgtgga gccatccgca gttcgaaaaa taagtcgacc
      721 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      781 agcataaccc cttggg
//
