LOCUS       sequence-125348-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJL1 expression vector with sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, 
            Majewska NI, Chen CX, Marcum RD, Weiss MG, Stoddart AE, Amiram M, 
            Ranji Charna AK, Patel JR, Isaacs FJ, Kelleher NL, Hong SH, Jewett 
            MC
  TITLE     Cell-free protein synthesis from genomically recoded bacteria 
            enables multisite incorporation of noncanonical amino acids.
  JOURNAL   Nat Commun. 2018 Mar 23;9(1):1203. doi: 10.1038/s41467-018-03469-5.
  PUBMED    29572528
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-23"; volume: "9(1):1203. doi"; pages: " 
            10.1038/s41467-018-03469-5"
COMMENT     Sequence Label: pJL1
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(97..144)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     140..158
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(213..236)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(237..284)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     RBS             940..962
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(993..1012)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(994..1012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
       61 gctcgatgag tttttctaat cagaattggc tttcagcaaa aaacccctca agacccgttt
      121 agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca actcagcttc
      181 ctttcgggct ttgttagcag ccggtcgact tatttttcga actgcggatg gctccacgta
      241 atacctgccg cattcacata ttcgtgcaga accatgtggt cccgcgtgcc tttttcgttc
      301 ggatctttag acagaacggt ctgcgtgctc agataatgat tatccggcag cagcaccgga
      361 ccatcaccaa tcggggtatt ctgctgataa tgatctgcca gctgcacact gccatcttca
      421 acgttatggc gaacggtaaa attcgctttg atgccatttt tctgtttatc cgccgtaata
      481 taaacattat ggctattaaa gttgtattcc agtttatggc ccaggatatt gccatcttct
      541 ttaaaatccg tgcctttcag ttcaatgcgg ttcaccaggg tatcgccttc aaatttcaca
      601 acggcgcgcg ttttatattt gccatcatct ttaaagctaa tcgtacgttc ctgcacatag
      661 ccttccggca ttgcagattt aaagaaatcg tgacgtttca tgtgatccgg atagcgacta
      721 aaacactgaa cgccataggt cagggtggtc accagcgtcg gccacggcac cggcagtttg
      781 ccggtggtgc agataaattt cagcgtcagt ttgccaatcg tggcatcgcc ttcaccttca
      841 ccacgcacgc tgaatttgtg accgttcaca tcgccatcca gttccaccag aatcggcaca
      901 acgccggtaa acagttcttc acctttgctc atatgtatat ctccttctta aagttaaaca
      961 aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg
     1021 ggatcgcgtt gctggcgttt ttccataggc tccgcccccc
//
