LOCUS       sequence-125351-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ribo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS
  TITLE     Protein synthesis by ribosomes with tethered subunits.
  JOURNAL   Nature. 2015 Jul 29. doi: 10.1038/nature14862.
  PUBMED    26222032
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jul 29. doi: 10.1038/nature14862."
COMMENT     Sequence Label: poRibo-T2
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             529..543
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
ORIGIN
        1 aacagcttcg gcgttgtaag cttaagctca caacccgaag atgtttcttt cgattcatca
       61 tcgtgttgcg aaaatttgag agactcacga acaactctcg ttgttcagtg tttcaatttt
      121 cagcttgatc cagattttta aagagcaaat atctcaaaca tcacccgaag atgagttttg
      181 agatattaag gtcggcgact ttcactcaca aaccagcaag tggcgtcccc taggggattc
      241 gaacccctgt taccgccgtg aaagggcggt gtcctgggcc tctagacgaa ggggacacga
      301 aaattgctta tcacgcgttg cgtgatattt tcgtgtaggg tgagctttca ttaatagaaa
      361 gcgaacggcc ttattctctt cagcctcact cccaacgcgt aaacgccttg cttttcactt
      421 tctatcagac aatctgtgtg agcactacaa agtacgcttc tttaaggtac cacaatgatc
      481 caaccgcagg ttcccctacg gttaccttgt tacgacttca ccccagtcat gaatcacaaa
      541 gtggtaagcg ccctcctttt tttttctgcg cttacacacc cggcctatca acgtcgtcgt
      601 cttcaacgtt ccttcaggac ccttaaaggg tcagggagaa ctcatctcgg ggcaagtttc
      661 gtgcttagat gctttcagca cttatctctt ccgcatttag ctaccgggca gtgccattgg
      721 catgacaacc cgaacaccag tgatgcgtcc actccggtcc tctcgtacta ggagcagccc
      781 ccctcagttc tccagcgccc acggcagata gggaccgaac tgtctcacga cgttctaaac
      841 ccagctcgcg taccacttta aatggcgaac agccataccc ttgggaccta cttcagcccc
      901 aggatgtgat gagccgacat cgaggtgcca aacaccgccg tcgatatgaa ctcttgggcg
      961 gtatcagcct gttatccccg gagtaccttt tatccgttga gcgatggccc ttccattcag
     1021 aaccaccgga tcac
//
