LOCUS       sequence-125353-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTET pduD1-20-mCherry-ssrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ206
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             114..125
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(332..350)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     600..619
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actttacttt tatctaatct agacatcatt aattcctaat tttgttgaca ctctatcggt
       61 tgatagagct tattttacca ctccctatca gtgatagaga aaagagaatt cttaaagagg
      121 agaaaggtca tggaaattaa tgaaaaattg ctgcgccaga taattgaaga cgtactccgc
      181 gatatgaagg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      241 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      301 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      361 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      421 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      481 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg
      541 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      601 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      661 gacggcgccc tgaagggcga gatcaag
//
