LOCUS       sequence-125355-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of GFP (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCMV-GFP
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    46..180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(70..89)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     124..143
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(186..381)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      200..335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    440..461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(476..481,482..499,500..506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    514..530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     519..541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     538..554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     538..554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     897..916
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    915..970
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1016..1035
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc agacatgata
       61 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      121 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      181 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
      241 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
      301 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
      361 gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac tggaaagcgg
      421 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      541 gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga tctccataag
      601 agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa
      661 acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa
      721 aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt
      781 ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca
      841 tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca
      901 tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat
      961 ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg
     1021 gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc
     1081 accttctgat aggcagcctg cacctgagga gtgcggccgc tttacttg
//
