LOCUS       sequence-125356-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Clone cassette to replace RFP for integration into C31 landing pad 
            strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Heil JR, Cheng J, Charles TC
  TITLE     Site-specific bacterial chromosome engineering: PhiC31 integrase 
            mediated cassette exchange (IMCE).
  JOURNAL   J Vis Exp. 2012 Mar 16;(61). pii: 3698. doi: 10.3791/3698.
  PUBMED    22453661
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2012-03-16"; volume: "(61). pii: 3698. doi"; pages: " 
            10.3791/3698"
COMMENT     Sequence Label: pJH110
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..36
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        43..77
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     RBS             86..97
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aggtgccagg gcgtgccctt gggctccccg ggcgcggcat gcttgacggc tagctcagtc
       61 ctaggtattg tgctagctac tagagaaaga ggagaaatac tagatggctt cctccgaaga
      121 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
      181 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
      241 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
      301 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
      361 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
      421 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
      481 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
      541 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
      601 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
      661 ggttcagctg ccgggtgctt acaa
//
