LOCUS       sequence-125357-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Clone cassette to replace RFP for integration into C31 landing pad 
            strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Heil JR, Cheng J, Charles TC
  TITLE     Site-specific bacterial chromosome engineering: PhiC31 integrase 
            mediated cassette exchange (IMCE).
  JOURNAL   J Vis Exp. 2012 Mar 16;(61). pii: 3698. doi: 10.3791/3698.
  PUBMED    22453661
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2012-03-16"; volume: "(61). pii: 3698. doi"; pages: " 
            10.3791/3698"
COMMENT     Sequence Label: pJH110
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(152..685)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ctcccgggcg cgtgaaataa gtggctgcgc gcataaagcg ggtgatggga ctaacgcctg
       61 agttaagccg gagcgctttg cggccgcggc gttgtgacaa tttaccgaac aactccgcgg
      121 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
      181 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
      241 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
      301 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
      361 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
      421 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
      481 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
      541 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
      601 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
      661 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
      721 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtac
//
