LOCUS       sequence-125362-         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJH122 variant Gateway destination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJH123
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(508..555)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cggtgagttc aggctttttc atatcttgcc ccccgggatc tgttgtaagg agtctagagc
       61 ttggaggtct gattttctca gtctccagag atgtgtttat ataggtagta acctttttga
      121 tatcagccac aagtgtgtgg gaatcttatc ttcggatttc aattaggaat taaccttatt
      181 gaattctctt gaaaggaagt ccgcaaagtg gttgtcggtt cctttaatat gttcaacatc
      241 aaacgaatag tggctaagcc atgcttgcca tctgatgttt cttccaagtt ttgaatctcc
      301 tttgtaatta aggttaacaa aactcttgaa atgagtatta tctgtcctaa tcagaaaatg
      361 aacaggagtt agataaatac tgaatttctt tatagtattt attaccgcca atgtctcttt
      421 gtcattgctg tggtaattcg gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
      481 agctcgaatt agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
      541 cggaatagga acttcaagat cccctgattc cctttgtcaa cagcaatgga tatc
//
